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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Immunocytochemistry [1]
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- Product number
- MA5-32160 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PRMT5 Recombinant Rabbit Monoclonal Antibody (ST51-06)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- ST51-06
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Protein Arginine Methyltransferase 5 as a Therapeutic Target for KRAS Mutated Colorectal Cancer.
Shifteh D, Sapir T, Pahmer M, Haimowitz A, Goel S, Maitra R
Cancers 2020 Jul 28;12(8)
Cancers 2020 Jul 28;12(8)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of PRMT5 in HepG2 cells using a PRMT5 Monoclonal antibody (Product # MA5-32160) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 PRMT5 is shown to be further overexpressed in the KRAS mutant CRC cells at the translational level by Western blot analysis. ( a ) Western blot assay results displaying PRMT5 and corresponding beta-actin bands; ( b ) Quantified Western blot assay results showing that PRMT5 protein is 4.8-Fold ( p < 0.01) further overexpressed in the KRAS mutant CRC cells when compared to the KRAS WT CRC cells. Data are expressed as means + SD from two independent experiments using four CRC cell lines (2 KRAS mutant, 2 KRAS WT), as well as one normal colon cell line. The uncropped Western blot membranes can be seen in Figure S1 . Data for the individual cell lines can be seen in Figures S2 and S3 . * represents p < 0.05; ** represents p < 0.01.
