Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
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Validation data
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- Product number
- PA1-41390 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PRMT5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Suggested positive control: HeLa, NIH-3T3, Hela whole cell extract, antigen standard for PRMT5 (transient overexpression lysate).
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PRMT5 in A) human HeLa and B) mouse NIH 3T3 lysate using a PRMT5 polyclonal antibody (Product # PA1-41390) at 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PRMT5 in 20 µg HeLa cell lysates. Samples were incubated in PRMT5 polyclonal antibody (Product # PA1-41390) using a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PRMT5 in A) human HeLa and B) mouse NIH 3T3 lysate. Samples were incubated in PRMT5 polyclonal antibody (Product # PA1-41390).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using PRMT5 Polyclonal Antibody (Product # PA1-41390) and bands of ~ 55, 66 kDa corresponding to PRMT5 was observed across all the cell lines tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), MCF-7 (Lane 2), T47D (Lane 3), Hep G2 (Lane 4) and NIH/3T3 (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).