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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Other assay [2]
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- Product number
- PA5-30383 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PRMT5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, Jurkat, Raji.
- Concentration
- 0.49 mg/mL
Submitted references A PRMT5-RNF168-SMURF2 Axis Controls H2AX Proteostasis.
Du C, Hansen LJ, Singh SX, Wang F, Sun R, Moure CJ, Roso K, Greer PK, Yan H, He Y
Cell reports 2019 Sep 17;28(12):3199-3211.e5
Cell reports 2019 Sep 17;28(12):3199-3211.e5
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PRMT5 Polyclonal Antibody (Product # PA5-30383). Sample (30 µg of whole cell lysate). Lane A: Raji. 7.5% SDS PAGE. SKB1 antibody. PRMT5 Polyclonal Antibody (Product # PA5-30383) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PRMT5 in paraformaldehyde-fixed A431 cells using a PRMT5 polyclonal antibody (Product # PA5-30383) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Mahlavu xenograft, using PRMT5 (Product # PA5-30383) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PRMT5 Polyclonal Antibody detects PRMT5 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. PRMT5 stained by PRMT5 Polyclonal Antibody (Product # PA5-30383) diluted at 1:2,500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PRMT5 Polyclonal Antibody detects PRMT5 protein at cytosol and nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. PRMT5 Polyclonal Antibody (Product # PA5-30383) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PRMT5 Polyclonal Antibody detects PRMT5 protein at cytosol and nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. PRMT5 Polyclonal Antibody (Product # PA5-30383) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
