Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-30576 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PRMT5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, Raji. Predicted reactivity: Mouse (98%), Rat (97%), Pig (98%), Rhesus Monkey (100%), Chimpanzee (99%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.52 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PRMT5 Polyclonal Antibody (Product # PA5-30576). Sample (30 µg of whole cell lysate). Lane A: Raji . 7.5% SDS PAGE. PRMT5 Polyclonal Antibody (Product # PA5-30576) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PRMT5 in paraformaldehyde-fixed A431 cells using a PRMT5 polyclonal antibody (Product # PA5-30576) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using PRMT5 (Product # PA5-30576) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PRMT5 Polyclonal Antibody detects PRMT5 protein at cytosol on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. PRMT5 Polyclonal Antibody (Product # PA5-30576) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PRMT5 Polyclonal Antibody detects PRMT5 protein at cytosol on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. PRMT5 Polyclonal Antibody (Product # PA5-30576) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.