Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX628488 - Provider product page
- Provider
- GeneTex
- Product name
- PRMT5 antibody [GT3610]
- Antibody type
- Monoclonal
- Reactivity
- Human, Rat
- Host
- Mouse
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PRMT5 antibody detects PRMT5 protein by western blot analysis.A. 30 ?g Jurkat whole cell lysate/extractB. 30 ?g Raji whole cell lysate/extractC. 30 ?g K562 whole cell lysate/extractD. 30 ?g THP-1 whole cell lysate/extractE. 30 ?g HL-60 whole cell lysate/extractF. 30 ?g NCI-H929 whole cell lysate/extract7.5% SDS-PAGEPRMT5 antibody (GTX628488) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PRMT5 antibody [GT3610] detects PRMT5 protein by western blot analysis.A. 50 ?g rat kidney lysate/extract7.5% SDS-PAGEPRMT5 antibody [GT3610] (GTX628488) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PRMT5 antibody [GT3610] immunoprecipitates PRMT5 protein in IP experiments.IP samples: Raji whole cell extractA. 35 £gg Raji whole cell extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of PRMT5 protein by 4 £gg PRMT5 antibody [GT3610] (GTX628488)7.5 % SDS-PAGEThe immunoprecipitated PRMT5 protein was detected by PRMT5 antibody [GT3610] (GTX628488) diluted at 1:1000.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
