TA504680

antibody from OriGene

Targeting: UBE2E3 UbcH9

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

TA504680

Provider

OriGene

Proper citation

OriGene Cat#TA504680, RRID:AB_2622520

Product name

UBE2E3 mouse monoclonal antibody, clone OTI1A3 (formerly 1A3)

Antibody type

Monoclonal

Description

UBE2E3 mouse monoclonal antibody, clone OTI1A3 (formerly 1A3)

Host

Mouse

Conjugate

Unconjugated

Epitope

UBE2E3

Isotype

IgG

Antibody clone number

OTI1A3

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-UBE2E3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2E3 (RC210328, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2E3.

Validation comment

WB