PAB29537

antibody from Abnova Corporation

Targeting: UBE2E3 UbcH9

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29537

Provider

Abnova Corporation

Product name

UBE2E3 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human UBE2E3.

Antigen sequence

SSDRQRSDDESPSTSSGSSDADQRDPAAPEPEEQE
ERKPSATQQKKNTKLSSKTTAKLSTSAKRIQKELA
EITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYE
GGVFFLDITFSSDYPFKPPKVTFRTRIYHCNINSQ
GV


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with UBE2E3 polyclonal antibody (Cat # PAB29537).

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with UBE2E3 polyclonal antibody (Cat # PAB29537) shows cytoplasmic and nuclear positivity in cells in seminiferus ducts at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)