TA504683

antibody from OriGene

Targeting: UBE2E3 UbcH9

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA504683

Provider

OriGene

Proper citation

OriGene Cat#TA504683, RRID:AB_2622523

Product name

UBE2E3 mouse monoclonal antibody, clone OTI4G4 (formerly 4G4)

Antibody type

Monoclonal

Description

UBE2E3 mouse monoclonal antibody, clone OTI4G4 (formerly 4G4)

Host

Mouse

Conjugate

Unconjugated

Epitope

UBE2E3

Isotype

IgG

Antibody clone number

OTI4G4

Vial size

100 µl

Concentration

0.7 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2E3 (RC210328, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2E3.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-UBE2E3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC210328 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-UBE2E3 antibody(TA504683), and then analyzed by flow cytometry.

Validation comment

FC