Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-29687 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UBC13 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, HepG2, mouse brain. Predicted reactivity: Mouse (100%), Rat (99%), Xenopus laevis (97%), Dog (100%), Chicken (99%), Sheep (100%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using UBC13 Polyclonal Antibody (Product # PA5-29687). Sample (30 µg of whole cell lysate). Lane A: A431. Lane B: Hep G2. 12% SDS PAGE. UBC13 Polyclonal Antibody (Product # PA5-29687) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using UBC13 Polyclonal Antibody (Product # PA5-29687). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. UBC13 Polyclonal Antibody (Product # PA5-29687) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of UBE2N was achieved by transfecting Hep G2 with UBE2N specific siRNAs (Silencer® select Product # s14596, s14595). Western blot analysis (Fig. a) was performed using whole cell extracts from the UBE2N knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with UBC13 Polyclonal Antibody (Product # PA5-29687, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to UBE2N. An uncharacterized band (*) was observed at ~80 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using anti-UBC13 Polyclonal Antibody (Product # PA5-29687) and a 17kDa band corresponding to UBE2N was observed across cell lines and tissue extracts along with uncharacterized bands (*) at ~80 kDa and ~40 kDa. Whole cell extracts (30 µg lysate) of NIH:OVCAR-3 (Lane 1), BeWo (Lane 2), HL60 (Lane 3), MOLT-4 (Lane 4), tissue extracts of Mouse Testis (Lane 5), Mouse Brain (Lane 6), Mouse Liver (Lane 7) and Mouse Ovary (Lane 8) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- UBC13 Polyclonal Antibody detects UBE2N protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: UBE2N protein stained by UBC13 Polyclonal Antibody (Product # PA5-29687) diluted at 1:500. Blue: Hoechst 33342 staining.