PA5-29687

antibody from Invitrogen Antibodies

Targeting: UBE2N MGC8489, UBC13, UbcH-ben

Antibody data

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Validation data

Product number: PA5-29687 - Provider product page
Provider: Invitrogen Antibodies
Product name: UBC13 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: A431, HepG2, mouse brain. Predicted reactivity: Mouse (100%), Rat (99%), Xenopus laevis (97%), Dog (100%), Chicken (99%), Sheep (100%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

UBE2N protein structure - PA5-29687 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using UBC13 Polyclonal Antibody (Product # PA5-29687). Sample (30 µg of whole cell lysate). Lane A: A431. Lane B: Hep G2. 12% SDS PAGE. UBC13 Polyclonal Antibody (Product # PA5-29687) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using UBC13 Polyclonal Antibody (Product # PA5-29687). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. UBC13 Polyclonal Antibody (Product # PA5-29687) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of UBE2N was achieved by transfecting Hep G2 with UBE2N specific siRNAs (Silencer® select Product # s14596, s14595). Western blot analysis (Fig. a) was performed using whole cell extracts from the UBE2N knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with UBC13 Polyclonal Antibody (Product # PA5-29687, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to UBE2N. An uncharacterized band (*) was observed at ~80 kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using anti-UBC13 Polyclonal Antibody (Product # PA5-29687) and a 17kDa band corresponding to UBE2N was observed across cell lines and tissue extracts along with uncharacterized bands (*) at ~80 kDa and ~40 kDa. Whole cell extracts (30 µg lysate) of NIH:OVCAR-3 (Lane 1), BeWo (Lane 2), HL60 (Lane 3), MOLT-4 (Lane 4), tissue extracts of Mouse Testis (Lane 5), Mouse Brain (Lane 6), Mouse Liver (Lane 7) and Mouse Ovary (Lane 8) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).