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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-54184 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CWC27 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Immunogen sequence: REDQTLALLN QFKSKLTQAI AETPENDIPE TEVEDDEGWM SHVLQFEDKS RKVKDASMQD SDTFEIYDPR NPVNKRRREE SK Highest antigen sequence identity to the following orthologs: Mouse - 93%, Rat - 90%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 0.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CWC27 in human cell line HEK 293 using a CWC27 Polyclonal Antibody (Product # PA5-54184).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CWC27 in mouse cell line NIH-3T3 and rat cell line NBT-II using a CWC27 Polyclonal Antibody (Product # PA5-54184).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of CWC27 in human cell line U-251 MG shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a CWC27 Polyclonal Antibody (Product # PA5-54184).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorecent analysis of CWC27 in human cell line U-251 MG using CWC27 Polyclonal Antibody (Product # PA5-54184). Staining shows localization to nucleoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CWC27 in human esophagus using CWC27 Polyclonal Antibody (Product # PA5-54184) shows strong nuclear positivity in squamous epithelial cells.
