PA5-55172

antibody from Invitrogen Antibodies

Targeting: HOOK3 HK3

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-55172

Provider

Invitrogen Antibodies

Product name

HOOK3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: KEEIAQRCHE LDMQVAALQE EKSSLLAENQ VLMERLNQSD SIEDPNSPAG RRHLQLQTQL EQLQEETFRL EA Highest antigen sequence identity to the following orthologs: Mouse - 99%, Rat - 58%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Hook3 is a scaffold for the opposite-polarity microtubule-based motors cytoplasmic dynein-1 and KIF1C.

Kendrick AA, Dickey AM, Redwine WB, Tran PT, Vaites LP, Dzieciatkowska M, Harper JW, Reck-Peterson SL
The Journal of cell biology 2019 Sep 2;218(9):2982-3001

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of HOOK3 in human cell line U-251 MG shows positivity in cytoplasm & microtubule organizing center. Samples were probed using a HOOK3 Polyclonal Antibody (Product # PA5-55172).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6. KIF1C recruits Hook3 to the cell periphery. (A) 293T KIF1C KO cells (KO) were infected with viral particles encoding MSCV-driven KIF1C-tagRFP-3xFLAG or KIF1C Delta794-807 -tagRFP-3xFLAG plasmids. Immunoblots were performed with the indicated antibodies. Low and high exposures with the KIF1C antibody are shown. beta-Actin provided a loading control. 293T cells transfected with CRISPR-Cas9 (CTRL) were used as control cells. (B) Confocal microscopy of KIF1C and Hook3 localization in stable 293T cell lines expressing KIF1C-tagRFP-3xFLAG or KIF1C Delta794-807 -tagRFP-3xFLAG. Cells were grown on glass coverslips, fixed, and stained for endogenous Hook3 (Endo-Hook3). The tagRFP and Hook3 signals are shown in representative maximum intensity projections. The overlap of intensity profiles (AU) generated from drawing a 15-um line segment across individual z-sections is shown to the right of the images. (C) The mean normalized Hook3 intensity within KIF1C foci for KIF1C-tagRFP-3xFLAG ( n = 25) or KIF1C Delta794-807 -tagRFP-3xFLAG ( n = 24). Foci were determined by thresholding the KIF1C image, and masks of these foci were used to measure the Hook3 intensity in the corresponding regions in maximum projection images. Box plots represent the maximum and minimum values. Statistical significance was calculated with an unpaired t test. ****, P < 0.0001. Representative data from three independent experiments is shown. (D) Confocal microscopy of KIF1C and Hook3 in U2OS cel