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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AF6216 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TRIF/TICAM1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human TRIF/TICAM1 in Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q8IUC6- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Differences in PGE2 production between primary human monocytes and differentiated macrophages: role of IL-1β and TRIF/IRF3.
Enterovirus 68 3C protease cleaves TRIF to attenuate antiviral responses mediated by Toll-like receptor 3.
TRIM38 negatively regulates TLR3-mediated IFN-β signaling by targeting TRIF for degradation.
Endo Y, Blinova K, Romantseva T, Golding H, Zaitseva M
PloS one 2014;9(5):e98517
PloS one 2014;9(5):e98517
Enterovirus 68 3C protease cleaves TRIF to attenuate antiviral responses mediated by Toll-like receptor 3.
Xiang Z, Li L, Lei X, Zhou H, Zhou Z, He B, Wang J
Journal of virology 2014 Jun;88(12):6650-9
Journal of virology 2014 Jun;88(12):6650-9
TRIM38 negatively regulates TLR3-mediated IFN-β signaling by targeting TRIF for degradation.
Xue Q, Zhou Z, Lei X, Liu X, He B, Wang J, Hung T
PloS one 2012;7(10):e46825
PloS one 2012;7(10):e46825
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human TRIF/TICAM1 by Western Blot. Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line. PVDF Membrane was probed with 0.25 µg/mL of Human TRIF/TICAM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6216) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TRIF/TICAM1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TRIF/TICAM1 in Raji Human Cell Line. TRIF/TICAM1 was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Human TRIF/TICAM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6216) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
