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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PAB10306 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10306, RRID:AB_1713277
- Product name
- RSL1D1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of RSL1D1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Identification of a novel nucleus protein involved in the regulation of urokinase in 95D cells.
Suppression subtractive hybridization and expression profiling identifies a unique set of genes overexpressed in non-small-cell lung cancer.
Identification of differentially expressed genes in human trophoblast cells by differential-display RT-PCR.
Tong C, Tan L, Li P, Zhu YS
Acta biochimica et biophysica Sinica 2005 May;37(5):303-9
Acta biochimica et biophysica Sinica 2005 May;37(5):303-9
Suppression subtractive hybridization and expression profiling identifies a unique set of genes overexpressed in non-small-cell lung cancer.
Petroziello J, Yamane A, Westendorf L, Thompson M, McDonagh C, Cerveny C, Law CL, Wahl A, Carter P
Oncogene 2004 Oct 7;23(46):7734-45
Oncogene 2004 Oct 7;23(46):7734-45
Identification of differentially expressed genes in human trophoblast cells by differential-display RT-PCR.
Huch G, Hohn HP, Denker HW
Placenta 1998 Nov;19(8):557-67
Placenta 1998 Nov;19(8):557-67
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using RSL1D1 polyclonal antibody (Cat # PAB10306) shows detection of over-expressed RSL1D1 in lysates from HeLa cells transfected with Flag-RSL1D1.Lanes 1 and 3 contain lysate from Flag-RSL1D1 transfected HeLa cells.Lanes 2 and 4 contain lysate from cells transfected with null vector.Lanes 1 and 2 were blotted with anti-Flag antibody.Lanes 3 and 4 were probed with a 1 : 500 dilution of RSL1D1 polyclonal antibody.The band at 75 KDa, indicated by the arrowhead, corresponds to RSL1D1.Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence microscopy of HeLa cells transfected with RSL1D1 polyclonal antibody (Cat # PAB10306) .In the overlay, specific antibody staining is shown to co-localize with recombinant protein.Cells were fixed with methanol prior to staining.Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.
- Validation comment
- Immunofluorescence
