AP09369PU-N

antibody from Acris Antibodies GmbH

Targeting: RSL1D1 CSIG, DKFZP564M182, L12, PBK1, UTP30

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

AP09369PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09369PU-N, RRID:AB_2035831

Product name

anti RSL1D1

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to an internal sequence of human PBK1

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

1.1 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using affinity purified anti-PBK1 antibody shows detection of over-expressed PBK1 in lysates from HeLa cells transfected with Flag-PBK1. Lanes 1 and 3 contain lysate from Flag-PBK1 transfected HeLa cells. Lanes 2 and 4 contain lysate from cells transfected with null vector. Lanes 1 and 2 were blotted with anti-Flag antibody. Lanes 3 and 4 were probed with a 1:500 dilution of anti-PBK1. The band at 75 kDa, indicated by the arrowhead, corresponds to PBK1.

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunofluorescence microscopy of HeLa cells transfected with GFP-PBK1. In the overlay, specific antibody staining is shown to co-localize with recombinant protein. Cells were fixed with methanol prior to staining