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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
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Validation data
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- Product number
- NBP2-59196 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal JMJD2B Antibody
- Antibody type
- Polyclonal
- Description
- Whole Antiserum.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: JMJD2B Antibody [NBP2-59196] - Nuclear extracts of HeLa cells (40 ug) were analysed by Western blot using the antibody against JMJD2b diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: JMJD2B Antibody [NBP2-59196] - To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human JMJD2b . The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:3,000.
