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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- MA5-35692 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- JMJD2B Recombinant Rabbit Monoclonal Antibody (8I0I1)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Immunogen sequence: PPKEWKPRQT YDDIDDVVIP APIQQVVTGQ SGLFTQYNIQ KKAMTVGEYR RLANSEKYCT PRHQDFDDLE RKYWKNLTFV SPIYGADISG SLYDDDVAQW N
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 8I0I1
- Vial size
- 100 µL
- Concentration
- 1.5 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of JMJD2B in C6 cells. Samples were incubated with JMJD2B Monoclonal antibody (Product # MA5-35692) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of JMJD2B in U-2 OS cells. Samples were incubated with JMJD2B Monoclonal antibody (Product # MA5-35692) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of JMJD2B in 300 μg extracts of HCT116 cells. Samples were precipitated with 3 μg JMJD2B Monoclonal antibody (Product # MA5-35692). Western blot was performed from the immunoprecipitate using JMJD2B Monoclonal antibody (Product # MA5-35692) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ChIP analysis of JMJD2B in extracts of 5637 cells. Samples were incubated with JMJD2B Monoclonal antibody (Product # MA5-35692) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
