Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-74605 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-74605, RRID:AB_1049025
- Product name
- Rabbit Polyclonal JMJD2B Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Histone H3K9 demethylase JMJD2B induces hepatic steatosis through upregulation of PPARĪ³2.
SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.
RNF8- and RNF168-dependent degradation of KDM4A/JMJD2A triggers 53BP1 recruitment to DNA damage sites.
The histone demethylase JMJD2B plays an essential role in human carcinogenesis through positive regulation of cyclin-dependent kinase 6.
The histone demethylases JMJD1A and JMJD2B are transcriptional targets of hypoxia-inducible factor HIF.
Kim JH, Jung DY, Nagappan A, Jung MH
Scientific reports 2018 Sep 13;8(1):13734
Scientific reports 2018 Sep 13;8(1):13734
SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.
Xu M, Moresco JJ, Chang M, Mukim A, Smith D, Diedrich JK, Yates JR 3rd, Jones KA
PLoS pathogens 2018 May;14(5):e1007071
PLoS pathogens 2018 May;14(5):e1007071
RNF8- and RNF168-dependent degradation of KDM4A/JMJD2A triggers 53BP1 recruitment to DNA damage sites.
Mallette FA, Mattiroli F, Cui G, Young LC, Hendzel MJ, Mer G, Sixma TK, Richard S
The EMBO journal 2012 Apr 18;31(8):1865-78
The EMBO journal 2012 Apr 18;31(8):1865-78
The histone demethylase JMJD2B plays an essential role in human carcinogenesis through positive regulation of cyclin-dependent kinase 6.
Toyokawa G, Cho HS, Iwai Y, Yoshimatsu M, Takawa M, Hayami S, Maejima K, Shimizu N, Tanaka H, Tsunoda T, Field HI, Kelly JD, Neal DE, Ponder BA, Maehara Y, Nakamura Y, Hamamoto R
Cancer prevention research (Philadelphia, Pa.) 2011 Dec;4(12):2051-61
Cancer prevention research (Philadelphia, Pa.) 2011 Dec;4(12):2051-61
The histone demethylases JMJD1A and JMJD2B are transcriptional targets of hypoxia-inducible factor HIF.
Beyer S, Kristensen MM, Jensen KS, Johansen JV, Staller P
The Journal of biological chemistry 2008 Dec 26;283(52):36542-52
The Journal of biological chemistry 2008 Dec 26;283(52):36542-52
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: JMJD2B Antibody [NB100-74605] - Whole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Dilution used for WB at 0.5 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: JMJD2B Antibody [NB100-74605] - Detection of Human JMJD2B by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-JMJD2B antibody NB100-74605 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: JMJD2B Antibody [NB100-74605] - Detection of Human JMJD2B on HeLa whole cell lysate using NB100-74605. JMJD2B was also immunoprecipitated by rabbit anti-JMJD2B antibody NB100-74604.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: JMJD2B Antibody [NB100-74605] - Detection of human JMJD2B by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-JMJD2B antibody NB100-74605 (lot NB100-74605-2) used for IP at 6 ug/mg lysate. JMJD2B was also immunoprecipitated by a previous lot (lot NB100-74605-1) of this antibody For blotting immunoprecipitated JMJD2B, NB100-74605 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
