Explore
Validate
Learn
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF1207 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-17RB Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human IL-17 RB in Western blots. In Western blots, approximately 35% cross-reactivity with recombinant mouse (rm) IL-17 RB is observed, approximately 5% cross-reactivity with rmIL-17 R is observed, and less than 1% cross-reactivity with recombinant human IL-17 R is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
NP_061195- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Interleukin-25-induced chemokines and interleukin-6 release from eosinophils is mediated by p38 mitogen-activated protein kinase, c-Jun N-terminal kinase, and nuclear factor-kappaB.
Wong CK, Cheung PF, Ip WK, Lam CW
American journal of respiratory cell and molecular biology 2005 Aug;33(2):186-94
American journal of respiratory cell and molecular biology 2005 Aug;33(2):186-94
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-17 RB in Human Kidney. IL-17 RB was detected in immersion fixed paraffin-embedded sections of human kidney using 15 µg/mL Goat Anti-Human IL-17 RB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1207) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IL-17 RB in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Goat Anti-Human IL-17 RB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1207, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
