PA5-143837

antibody from Invitrogen Antibodies

Targeting: TMEM41B KIAA0033

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-143837

Provider

Invitrogen Antibodies

Product name

TMEM41B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Predicted species reactivity based on immunogen sequence: Horse (100%); Chimpanzee (100%).

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TMEM41B in Rat thymus and testis. Samples were incubated with TMEM41B Polyclonal antibody (Product # PA5-143837) using a dilution of 2 µg/mL followed by Goat anti-rabbit IgG HRP conjugate secondary antibody at a dilution of 1:10,000. 1h incubation at RT in 5% NFDM/TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TMEM41B in A549, HeLa, and HepG2. Samples were incubated with TMEM41B Polyclonal antibody (Product # PA5-143837) using a dilution of 2 µg/mL followed by Goat anti-rabbit IgG HRP conjugate secondary antibody at a dilution of 1:10,000. 1 h incubation at RT in 5% NFDM/TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TMEM41B in Mouse thymus and testis. Samples were incubated with TMEM41B Polyclonal antibody (Product # PA5-143837) using a dilution of 2 µg/mL followed by Goat anti-rabbit IgG HRP conjugate secondary antibody at a dilution of 1:10,000. 1h incubation at RT in 5% NFDM/TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of TMEM41B in 4% paraformaldehyde-fixed HeLa cells. Samples were incubated in TMEM41B Polyclonal antibody (Product # PA5-143837) using a dilution of 20 µg/mL followed by goat anti-rabbit IgG secondary antibody at a 1:500 dilution (green) and DAPI staining (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Immunofluorescent) analysis of TMEM41B in 4% paraformaldehyde-fixed human colon. Samples were incubated in TMEM41B Polyclonal antibody (Product # PA5-143837) using a dilution of 20 µg/mL followed by goat anti-rabbit IgG secondary antibody at a 1:500 dilution (green) and DAPI staining (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Immunofluorescent) analysis of TMEM41B in 4% paraformaldehyde-fixed rat lung. Samples were incubated in TMEM41B Polyclonal antibody (Product # PA5-143837) using a dilution of 20 µg/mL followed by goat anti-rabbit IgG secondary antibody at a 1:500 dilution (green) and DAPI staining (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Immunofluorescent) analysis of TMEM41B in 4% paraformaldehyde-fixed mouse kidney. Samples were incubated in TMEM41B Polyclonal antibody (Product # PA5-143837) using a dilution of 20 µg/mL followed by goat anti-rabbit IgG secondary antibody at a 1:500 dilution (green) and DAPI staining (blue).