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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Other assay [2]
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- Product number
- MA5-41061 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- C1QBP Monoclonal Antibody (04)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 04
- Vial size
- 100 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of Human C1QBP in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.5% Triton X-100 in PBS, blocked with 10% serum, and incubated with C1QBP Monoclonal Antibody (04) (Product # MA5-41061, 1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of C1QBP in Lane A: 0.5 mg Hela Whole Cell lysate, Lane B: 0.5 mg HepG2 Whole Cell Lysate. Samples were treated with 15 μl of 50 % Protein G agarose, incubated with monoclonal antibody (Product # MA5-41061) with a dilution of 1:100 , followed by Dylight 800-labeled antibody to Mouse IgG (H+L) using a dilution of 1:7,500. Assay was performed under reducing conditions. Predicted band size: 35 kDa, Observed band size: 35 kDa .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- C1QBP Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate, Lane B: 0.5 mg HepG2 Whole Cell Lysate 2 µL with C1QBP Monoclonal Antibody (04) (Product # MA5-41061) and 15 µL of 50 % Protein G agarose. Primary antibody: C1QBP Monoclonal Antibody (04), at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7,500 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 35 kDa.
