MA5-44926

antibody from Invitrogen Antibodies

Targeting: C1QBP gC1Q-R, gC1qR, HABP1, p32, SF2p32

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-44926

Provider

Invitrogen Antibodies

Product name

C1QBP Monoclonal Antibody (A8F4)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

A8F4

Vial size

100 µL

Concentration

1.17 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GC1q R in paraffin-embedded human kidney tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with GC1q R Monoclonal antibody (Product # MA5-44926) using a dilution of 1:1,200 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GC1q R in paraffin-embedded human large intestine tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with GC1q R Monoclonal antibody (Product # MA5-44926) using a dilution of 1:1,200 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of GC1q R in A549 cells. Cells were fixed and permeabilized then stained with GC1q R Monoclonal antibody (Product # MA5-44926) using a dilution of 1 µg/mL (red) at 4℃ for an hour followed by iFluor™ 488 conjugate-Goat anti-Mouse IgG secondary antibody at a dilution of 1:1,000 for 30 minutes at 4℃. Mouse IgG1 Isotype Control (green). Unlabeled sample was used as a control (cells without incubation with primary antibody; black).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of GC1q R in A549 cells. Cells were fixed and permeabilized then stained with GC1q R Monoclonal antibody (Product # MA5-44926) using a dilution of 1 µg/mL (red) at 4℃ for an hour followed by iFluor™ 488 conjugate-Goat anti-Mouse IgG secondary antibody at a dilution of 1:1,000 for 30 minutes at 4℃. Mouse IgG1 Isotype Control (green). Unlabeled sample was used as a control (cells without incubation with primary antibody; black).