PA5-14988

antibody from Invitrogen Antibodies

Targeting: C1QBP gC1Q-R, gC1qR, HABP1, p32, SF2p32

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-14988

Provider

Invitrogen Antibodies

Product name

C1QBP Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with bovine, mouse and rat based on sequence homology.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Phylogenetically Defined Isoforms of Listeria monocytogenes Invasion Factor InlB Differently Activate Intracellular Signaling Pathways and Interact with the Receptor gC1q-R.

Chalenko Y, Kalinin E, Marchenkov V, Sysolyatina E, Surin A, Sobyanin K, Ermolaeva S
International journal of molecular sciences 2019 Aug 24;20(17)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis using a GC1qR polyclonal antibody (Product # PA5-14988) in 293 cell lysates (35 µg per lane).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 In vitro study of interactions between idInlBs and gC1q-R using solid-phase microplate binding and competition assay. Microtiter plate wells were coated with recombinant human gC1q-R. Purified idInlBs were added. ELISA assay was performed with His-tag-specific polyclonal antibodies and HRP-conjugated secondary antibodies. ( a ) Saturation curve demonstrating binding of three idInlB isoforms to gC1q-R; purified idInlBs were added in concentrations 0-40 ug/mL: circles-idInlB9, triangles-idInlB13, squares-idInlB14; ( b ) a competition assay; polyclonal antibodies developed against oligopeptides specific for central (amino acids 76-104) and C-terminal (amino acids 221-249) parts of gC1q-R were added at concentration of 4 ug/mL 1 h before idInlBs were added in concentration 0.4 ug/mL. BSA was used as a negative control, and idInlBs without antibodies were used as positive controls. Mean +- SD from three experiments made in triplicate are shown; * p < 0.05; ** p < 0.01. Statistical significance of competition experiments relative to a corresponding positive control is shown.