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- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-14989 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- C1QBP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Phylogenetically Defined Isoforms of Listeria monocytogenes Invasion Factor InlB Differently Activate Intracellular Signaling Pathways and Interact with the Receptor gC1q-R.
Chalenko Y, Kalinin E, Marchenkov V, Sysolyatina E, Surin A, Sobyanin K, Ermolaeva S
International journal of molecular sciences 2019 Aug 24;20(17)
International journal of molecular sciences 2019 Aug 24;20(17)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a C1QBP polyclonal antibody (Product # PA5-14989) in HepG2 cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 In vitro study of interactions between idInlBs and gC1q-R using solid-phase microplate binding and competition assay. Microtiter plate wells were coated with recombinant human gC1q-R. Purified idInlBs were added. ELISA assay was performed with His-tag-specific polyclonal antibodies and HRP-conjugated secondary antibodies. ( a ) Saturation curve demonstrating binding of three idInlB isoforms to gC1q-R; purified idInlBs were added in concentrations 0-40 ug/mL: circles-idInlB9, triangles-idInlB13, squares-idInlB14; ( b ) a competition assay; polyclonal antibodies developed against oligopeptides specific for central (amino acids 76-104) and C-terminal (amino acids 221-249) parts of gC1q-R were added at concentration of 4 ug/mL 1 h before idInlBs were added in concentration 0.4 ug/mL. BSA was used as a negative control, and idInlBs without antibodies were used as positive controls. Mean +- SD from three experiments made in triplicate are shown; * p < 0.05; ** p < 0.01. Statistical significance of competition experiments relative to a corresponding positive control is shown.
