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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- LS-C312637 - Provider product page
- Provider
- LSBio
- Product name
- AIMP2 Antibody (aa298-320) LS-C312637
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Storage
- At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- WB of AIMP2 antibody. All lanes: Anti-AIMP2 at 0.5ug/ml. Lane 1: Rat Liver Tissue Lysate at 40ug. Lane 2: Rat Lung Tissue Lysate at 40ug. Lane 3: Rat Kidney Tissue Lysate at 40ug. Lane 4: Rat Brain Tissue Lysate at 40ug. Lane 5: JURKAT Whole Cell Lysate at 40ug. Lane 6: CEM Whole Cell Lysate at 40ug. Lane 7: HUT Whole Cell Lysate at 40ug. Lane 8: U93T Whole Cell Lysate at 40ug. Lane 9: U93T Whole Cell Lysate at 40ug. Predicted bind size: 35KD. Observed bind size: 35KD.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- AIMP2 antibody. IHC(P): Human Rectal Cancer Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC analysis of AIMP2 using anti-AIMP2 antibody. AIMP2 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-AIMP2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
