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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-31306 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- JTV-1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HCT116, HepG2, HeLa, A549. Predicted reactivity: Mouse (88%), Rat (90%), Pig (84%), Sheep (81%), Bovine (81%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Disease-associated mutations in a bifunctional aminoacyl-tRNA synthetase gene elicit the integrated stress response.
Jin D, Wek SA, Kudlapur NT, Cantara WA, Bakhtina M, Wek RC, Musier-Forsyth K
The Journal of biological chemistry 2021 Oct;297(4):101203
The Journal of biological chemistry 2021 Oct;297(4):101203
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using JTV-1 Polyclonal Antibody (Product # PA5-31306). Sample (30 µg of whole cell lysate). Lane A: HepG2. 10% SDS PAGE. JTV-1 Polyclonal Antibody (Product # PA5-31306) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of AIMP2 in paraformaldehyde-fixed HeLa cells using an AIMP2 polyclonal antibody (Product # PA5-31306) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- JTV-1 Polyclonal Antibody detects AIMP2 protein at cytosol on mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. JTV-1 Polyclonal Antibody (Product # PA5-31306) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 MSC association and protein integrity of EPRS-FLAG protein overexpressed in HEK293 T cells. A , doxycycline-inducible EPRS knockdown in HEK293T cells using shEPRS ( left ) or a nonspecific shRNA ( right ). B , immunoprecipitation experiments with FLAG antibody in HEK293T cells with endogenous EPRS knockdown and WT and mutant EPRS-FLAG overexpression. EPRS ( top ) and MSC scaffold proteins AIMP2 ( middle ) and AIMP3 ( bottom ) were visualized by immunoblotting using the indicated antibodies.
