PA5-88257

antibody from Invitrogen Antibodies

Targeting: TGFBR2 MFS2, TBR-ii, TBRII

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-88257

Provider

Invitrogen Antibodies

Product name

TGFBR2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Samples: Mouse spleen, Rat lung; Cellular Location: Cell membrane, Single-pass type I membrane protein Immunogen sequence: TIPPHVQKSV NNDMIVTDNN GAVKFPQLCK FCDVRFSTCD NQKSCMSNCS ITSICEKPQE VCVAVWRKND ENITLETVCH DPKLPYHDFI LEDAASPKCI MKEKKKPGET FFMCSCSSDE CNDNIIFSEE YNTSNPDLLL VIFQVTGISL LPPLGVAISV IIIFYCYRVN RQQKLSSTWE TGKTRKLMEF SEHCAIILED DRSDISSTCA NNINHNTELL PIELDTLVGK GRFAEVYKAK LKQNTSEQFE TVAVKIFP

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.82 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

References

Submitted references

A novel circ_MACF1/miR-942-5p/TGFBR2 axis regulates the functional behaviors and drug sensitivity in gefitinib-resistant non-small cell lung cancer cells.

Fan D, Yang Y, Zhang W
BMC pulmonary medicine 2022 Jan 7;22(1):27

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of U2OS cells using TGFBR2 Polyclonal antibody (Product # PA5-88257) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of TGFBR2 in paraffin-embedded rat spleen. Samples were incubated with TGFBR2 Polyclonal antibody (Product # PA5-88257) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of TGFBR2 in paraffin-embedded mouse spleen. Samples were incubated with TGFBR2 Polyclonal antibody (Product # PA5-88257) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of TGFBR2 in paraffin-embedded mouse spleen. Samples were incubated with TGFBR2 Polyclonal antibody (Product # PA5-88257) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 8 Overexpression of circ_MACF1 inhibits tumor growth under gefitinib in vivo. A qRT-PCR analysis of circ_MACF1 expression in A549/GR cells transduced by lentiviruses expressing circ_MACF1 (lenti-circ_MACF1) or a scrambled control (lenti-NC). A549/GR cells transduced by lenti-circ_MACF1 or lenti-NC were implanted into the right flanks of BALB/c nude mice (6 mice per group), with gefitinib administration (30 mg/kg) per os and tumor volume measurement ( B ) 10 days after implantation, and checked for tumor weight and images ( C ), circ_MACF1 expression by qRT-PCR analysis ( D ), miR-942-5p expression by qRT-PCR analysis ( E ), TGFBR2 mRNA level by qRT-PCR analysis ( F ), TGFBR2 protein level by western blot ( G ), TGFBR2 protein level and Ki67 staining by immunohistochemistry ( H ) at the end point (30 days after injection). * P < 0.05