APRab18856

antibody from EnkiLife Biotech Co., Ltd.

Targeting: TGFBR2 MFS2, TBR-ii, TBRII

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

APRab18856

Provider

EnkiLife Biotech Co., Ltd.

Product name

TGFβ RII Rabbit Polyclonal Antibody

Antibody type

Polyclonal

Description

Affinity purification

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

Polyclonal

Vial size

100 µl

Concentration

1 mg/ml

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Handling

The antibody solution should be gently mixed before use.

Western blot

Supportive validation

Submitted by

EnkiLife Biotech Co., Ltd. (provider)

Main image

Experimental details

Western blot analysis of lysates from HepG2 （65K） cells, using TGF beta Receptor II Antibody. The lane on the right is blocked with the synthesized peptide.

Supportive validation

Submitted by

EnkiLife Biotech Co., Ltd. (provider)

Main image

Experimental details

Western blot analysis of the lysates from HT-29 cells using TGF β Receptor II antibody.

Immunocytochemistry

Supportive validation

Submitted by

EnkiLife Biotech Co., Ltd. (provider)

Main image

Experimental details

Immunofluorescence analysis of rat-kidney tissue. 1,TGFβ RII Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Supportive validation

Submitted by

EnkiLife Biotech Co., Ltd. (provider)

Main image

Experimental details

Immunofluorescence analysis of rat-kidney tissue. 1,TGFβ RII Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Supportive validation

Submitted by

EnkiLife Biotech Co., Ltd. (provider)

Main image

Experimental details

Immunofluorescence analysis of rat-spleen tissue. 1,TGFβ RII Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Supportive validation

Submitted by

EnkiLife Biotech Co., Ltd. (provider)

Main image

Experimental details

Immunofluorescence analysis of rat-spleen tissue. 1,TGFβ RII Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Supportive validation

Submitted by

EnkiLife Biotech Co., Ltd. (provider)

Main image

Experimental details

Immunofluorescence analysis of mouse-kidney tissue. 1,TGFβ RII Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Supportive validation

Submitted by

EnkiLife Biotech Co., Ltd. (provider)

Main image

Experimental details

Immunofluorescence analysis of mouse-kidney tissue. 1,TGFβ RII Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B