Antibody data
- Antibody Data
- Antigen structure
- References [19]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF-241-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human TGF-beta RII Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human TGF-beta RII in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse TGF-beta RII is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P37173.2
- Isotype
- IgG
- Vial size
- 250 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Inhibition of IL-10 and TGF-β receptors on dendritic cells enhances activation of effector T-cells to kill cholangiocarcinoma cells.
TGF-β contamination of purified recombinant GDF15.
24R,25-Dihydroxyvitamin D3 Protects against Articular Cartilage Damage following Anterior Cruciate Ligament Transection in Male Rats.
Clathrin light chains' role in selective endocytosis influences antibody isotype switching.
Mesothelial cells promote early ovarian cancer metastasis through fibronectin secretion.
CD4+CD25+ T regulatory cells activated during feline immunodeficiency virus infection convert T helper cells into functional suppressors through a membrane-bound TGFβ / GARP-mediated mechanism.
Altered microenvironment promotes progression of preinvasive breast cancer: myoepithelial expression of αvβ6 integrin in DCIS identifies high-risk patients and predicts recurrence.
Integrin-mediated type II TGF-β receptor tyrosine dephosphorylation controls SMAD-dependent profibrotic signaling.
T-cell TGF-β signaling abrogation restricts medulloblastoma progression.
TGF-β mediates homing of bone marrow-derived human mesenchymal stem cells to glioma stem cells.
Targeting of SMAD5 links microRNA-155 to the TGF-beta pathway and lymphomagenesis.
Antibody-mediated blockade of integrin alpha v beta 6 inhibits tumor progression in vivo by a transforming growth factor-beta-regulated mechanism.
Tumor-induced anorexia and weight loss are mediated by the TGF-beta superfamily cytokine MIC-1.
Very low O2 concentration (0.1%) favors G0 return of dividing CD34+ cells.
Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.
TGF-beta1 regulates TGF-beta1 and FGF-2 mRNA expression during fibroblast wound healing.
TGF-beta1 regulates TGF-beta1 and FGF-2 mRNA expression during fibroblast wound healing.
Transforming growth factor-beta1 regulation of growth zone chondrocytes is mediated by multiple interacting pathways.
Transforming growth factor-beta1 regulation of growth zone chondrocytes is mediated by multiple interacting pathways.
Thepmalee C, Panya A, Junking M, Chieochansin T, Yenchitsomanus PT
Human vaccines & immunotherapeutics 2018 Jun 3;14(6):1423-1431
Human vaccines & immunotherapeutics 2018 Jun 3;14(6):1423-1431
TGF-β contamination of purified recombinant GDF15.
Olsen OE, Skjærvik A, Størdal BF, Sundan A, Holien T
PloS one 2017;12(11):e0187349
PloS one 2017;12(11):e0187349
24R,25-Dihydroxyvitamin D3 Protects against Articular Cartilage Damage following Anterior Cruciate Ligament Transection in Male Rats.
Boyan BD, Hyzy SL, Pan Q, Scott KM, Coutts RD, Healey R, Schwartz Z
PloS one 2016;11(8):e0161782
PloS one 2016;11(8):e0161782
Clathrin light chains' role in selective endocytosis influences antibody isotype switching.
Wu S, Majeed SR, Evans TM, Camus MD, Wong NM, Schollmeier Y, Park M, Muppidi JR, Reboldi A, Parham P, Cyster JG, Brodsky FM
Proceedings of the National Academy of Sciences of the United States of America 2016 Aug 30;113(35):9816-21
Proceedings of the National Academy of Sciences of the United States of America 2016 Aug 30;113(35):9816-21
Mesothelial cells promote early ovarian cancer metastasis through fibronectin secretion.
Kenny HA, Chiang CY, White EA, Schryver EM, Habis M, Romero IL, Ladanyi A, Penicka CV, George J, Matlin K, Montag A, Wroblewski K, Yamada SD, Mazar AP, Bowtell D, Lengyel E
The Journal of clinical investigation 2014 Oct;124(10):4614-28
The Journal of clinical investigation 2014 Oct;124(10):4614-28
CD4+CD25+ T regulatory cells activated during feline immunodeficiency virus infection convert T helper cells into functional suppressors through a membrane-bound TGFβ / GARP-mediated mechanism.
Miller MM, Petty CS, Tompkins MB, Fogle JE
Virology journal 2014 Jan 18;11:7
Virology journal 2014 Jan 18;11:7
Altered microenvironment promotes progression of preinvasive breast cancer: myoepithelial expression of αvβ6 integrin in DCIS identifies high-risk patients and predicts recurrence.
Allen MD, Thomas GJ, Clark S, Dawoud MM, Vallath S, Payne SJ, Gomm JJ, Dreger SA, Dickinson S, Edwards DR, Pennington CJ, Sestak I, Cuzick J, Marshall JF, Hart IR, Jones JL
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Jan 15;20(2):344-57
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Jan 15;20(2):344-57
Integrin-mediated type II TGF-β receptor tyrosine dephosphorylation controls SMAD-dependent profibrotic signaling.
Chen X, Wang H, Liao HJ, Hu W, Gewin L, Mernaugh G, Zhang S, Zhang ZY, Vega-Montoto L, Vanacore RM, Fässler R, Zent R, Pozzi A
The Journal of clinical investigation 2014 Aug;124(8):3295-310
The Journal of clinical investigation 2014 Aug;124(8):3295-310
T-cell TGF-β signaling abrogation restricts medulloblastoma progression.
Gate D, Danielpour M, Rodriguez J Jr, Kim GB, Levy R, Bannykh S, Breunig JJ, Kaech SM, Flavell RA, Town T
Proceedings of the National Academy of Sciences of the United States of America 2014 Aug 19;111(33):E3458-66
Proceedings of the National Academy of Sciences of the United States of America 2014 Aug 19;111(33):E3458-66
TGF-β mediates homing of bone marrow-derived human mesenchymal stem cells to glioma stem cells.
Shinojima N, Hossain A, Takezaki T, Fueyo J, Gumin J, Gao F, Nwajei F, Marini FC, Andreeff M, Kuratsu J, Lang FF
Cancer research 2013 Apr 1;73(7):2333-44
Cancer research 2013 Apr 1;73(7):2333-44
Targeting of SMAD5 links microRNA-155 to the TGF-beta pathway and lymphomagenesis.
Rai D, Kim SW, McKeller MR, Dahia PL, Aguiar RC
Proceedings of the National Academy of Sciences of the United States of America 2010 Feb 16;107(7):3111-6
Proceedings of the National Academy of Sciences of the United States of America 2010 Feb 16;107(7):3111-6
Antibody-mediated blockade of integrin alpha v beta 6 inhibits tumor progression in vivo by a transforming growth factor-beta-regulated mechanism.
Van Aarsen LA, Leone DR, Ho S, Dolinski BM, McCoon PE, LePage DJ, Kelly R, Heaney G, Rayhorn P, Reid C, Simon KJ, Horan GS, Tao N, Gardner HA, Skelly MM, Gown AM, Thomas GJ, Weinreb PH, Fawell SE, Violette SM
Cancer research 2008 Jan 15;68(2):561-70
Cancer research 2008 Jan 15;68(2):561-70
Tumor-induced anorexia and weight loss are mediated by the TGF-beta superfamily cytokine MIC-1.
Johnen H, Lin S, Kuffner T, Brown DA, Tsai VW, Bauskin AR, Wu L, Pankhurst G, Jiang L, Junankar S, Hunter M, Fairlie WD, Lee NJ, Enriquez RF, Baldock PA, Corey E, Apple FS, Murakami MM, Lin EJ, Wang C, During MJ, Sainsbury A, Herzog H, Breit SN
Nature medicine 2007 Nov;13(11):1333-40
Nature medicine 2007 Nov;13(11):1333-40
Very low O2 concentration (0.1%) favors G0 return of dividing CD34+ cells.
Hermitte F, Brunet de la Grange P, Belloc F, Praloran V, Ivanovic Z
Stem cells (Dayton, Ohio) 2006 Jan;24(1):65-73
Stem cells (Dayton, Ohio) 2006 Jan;24(1):65-73
Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.
Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB
Blood 2005 Jan 15;105(2):750-8
Blood 2005 Jan 15;105(2):750-8
TGF-beta1 regulates TGF-beta1 and FGF-2 mRNA expression during fibroblast wound healing.
Song QH, Klepeis VE, Nugent MA, Trinkaus-Randall V
Molecular pathology : MP 2002 Jun;55(3):164-76
Molecular pathology : MP 2002 Jun;55(3):164-76
TGF-beta1 regulates TGF-beta1 and FGF-2 mRNA expression during fibroblast wound healing.
Song QH, Klepeis VE, Nugent MA, Trinkaus-Randall V
Molecular pathology : MP 2002 Jun;55(3):164-76
Molecular pathology : MP 2002 Jun;55(3):164-76
Transforming growth factor-beta1 regulation of growth zone chondrocytes is mediated by multiple interacting pathways.
Rosado E, Schwartz Z, Sylvia VL, Dean DD, Boyan BD
Biochimica et biophysica acta 2002 Jun 12;1590(1-3):1-15
Biochimica et biophysica acta 2002 Jun 12;1590(1-3):1-15
Transforming growth factor-beta1 regulation of growth zone chondrocytes is mediated by multiple interacting pathways.
Rosado E, Schwartz Z, Sylvia VL, Dean DD, Boyan BD
Biochimica et biophysica acta 2002 Jun 12;1590(1-3):1-15
Biochimica et biophysica acta 2002 Jun 12;1590(1-3):1-15
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human TGF-beta RII by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Hep3B human hepatocellular carcinoma cell line, U-118-MG human glioblastoma/astrocytoma cell line, HT1080 human fibrosarcoma cell line, and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human TGF-beta RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-241-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TGF-beta RII at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TGF-beta RII in Human Pituitary. TGF-beta RII was detected in immersion fixed paraffin-embedded sections of human pituitary using Goat Anti-Human TGF-beta RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-241-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of TGF-beta RII in Human Lymphocytes by Flow Cytometry. Human blood-derived lymphocytes were labeled with Goat Anti-Human TGF-beta RII Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF-241-NA, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TGF-beta 1 Inhibition of IL-4-dependent Cell Proliferation and Neutralization by Human TGF-beta RII Antibody. Recombinant Human TGF-beta 1 (Catalog # 240-B) inhibits Recombinant Human IL-4 (Catalog # 204-IL) induced proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-4 (5 ng/mL) activity elicited by Recombinant Human TGF-beta 1 (0.04 ng/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti- Human TGF-beta RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-241-NA). The ND50 is typically 10-20 µg/mL.