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antibody from Invitrogen Antibodies
Targeting: SPEG
APEG1, BPEG, KIAA1297, MGC12676, MYLK6, SPEGalpha, SPEGbeta
Immunohistochemistry
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Other assay [1]
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- Product number
- PA5-53875 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SPEG Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: VYKSVIANKL GKAACYAHLY VTDVVPGPPD GAPQVVAVTG RMVTLTWNPP RSLDMAIDPD SLTYTVQHQV Highest antigen sequence identity to the following orthologs: Mouse - 97%, Rat - 99%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Characterization of a novel zebrafish model of SPEG-related centronuclear myopathy.
Striated Preferentially Expressed Protein Kinase (SPEG)-Deficient Skeletal Muscles Display Fewer Satellite Cells with Reduced Proliferation and Delayed Differentiation.
Espinosa KG, Geissah S, Groom L, Volpatti J, Scott IC, Dirksen RT, Zhao M, Dowling JJ
Disease models & mechanisms 2022 May 1;15(5)
Disease models & mechanisms 2022 May 1;15(5)
Striated Preferentially Expressed Protein Kinase (SPEG)-Deficient Skeletal Muscles Display Fewer Satellite Cells with Reduced Proliferation and Delayed Differentiation.
Li Q, Lin J, Rosen SM, Zhang T, Kazerounian S, Luo S, Agrawal PB
The American journal of pathology 2020 Dec;190(12):2453-2463
The American journal of pathology 2020 Dec;190(12):2453-2463
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 2. Speg expression during zebrafish development. There are two Speg genes in zebrafish, spega and spegb . Each encodes a single transcript that is highly conserved with human SPEG . (A,B) RT-qPCR shows similar temporal expression patterns of spega (A) versus spegb (B) from 2 days post-fertilization (dpf) to 7 dpf. Both Speg mRNA transcripts are significantly upregulated in the heads (light blue bars; 20-fold for spega , 7-fold for spegb ), but stay at relatively similar levels in the skeletal-muscle-predominant tails (white bars). Each data point represents the average of technical triplicates, and three independent experiments are included. Columns and error bars represent mean+-s.e.m. Unpaired two-tailed Student's t -test was performed: ** P
