20499-1-AP

antibody from Proteintech Group

Targeting: EIF2AK1 HRI, KIAA1369

Provider product page for 20499-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [26]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 20499-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#20499-1-AP, RRID:AB_10697665
Product name: EIF2AK1 antibody
Antibody type: Polyclonal
Description: EIF2AK1 antibody (Cat. #20499-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IHC, WB,ELISA.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

EIF2AK1 protein structure - 20499-1-AP shown in red.

Submitted references GABARAPL1 is important for the activation of HRI during eIF2α phosphorylation-dependent stress response to sodium arsenite.

Campenet S, Lamarre M, Durand J, Perez V, Peixoto P, Delage-Mourroux R, Guittaut M, Baguet A, Despouy G
Scientific reports 2026 Mar 28;16(1)

Trifloxystrobin induces oxidative stress-dependent activation of the OMA1-DELE1-HRI integrated stress response leading to apoptosis in human neuroblastoma cells.

Chaabani H, Ayed I, Rjiba K, Abid S, Eyer J, Arnoult D
Environmental pollution (Barking, Essex : 1987) 2026 Feb 1;390:127562

Ageing promotes metastasis via activation of the integrated stress response.

Patel AAH, Dzanan JJ, Ali KX, Eklund EA, Alvarez SW, Raj D, Dankis M, Altinönder I, Schwarz M, Le Gal K, Bedel E, El Zowalaty AE, Jonasson E, Albatrok H, Gul N, Bossowski JP, Pillai R, Micke P, Botling J, Akyürek LM, Angeletti D, Sayin SI, Härtlova A, Papagiannakopoulos T, Olofsson Bagge R, Ståhlberg A, Hallqvist A, Wiel C, Sayin VI
Nature 2026 Apr;652(8112):1339-1348

The integrated stress response suppresses PINK1-dependent mitophagy by preserving mitochondrial import efficiency.

Yang M, Mo Z, Walsh K, Liu W, Irahal IN, Arnoult D, Guo X
Nature communications 2026 Apr 9;

HSPA8 dampens SCAP/INSIG split and SREBP activation by reducing PKR-mediated INSIG phosphorylation.

Yang G, Ma C, Chen Y, Xiang J, Li L, Li Y, Kang L, Liang Z, Yang S
Cell reports 2025 Mar 25;44(3):115339

Endothelial GTPBP3 directs developmental angiogenesis and neovascularization after limb ischemia via the mtROS/HRl/ATF4/mTORC1 axis.

Qin D, Hu J, Yang Y, Li X, He J, Chen J, Guo X, Wei C, Wang F, Yi T, Li C, Yu B
Angiogenesis 2025 Jun 18;28(3):36

Molecular basis of SIFI activity in the integrated stress response.

Yang Z, Haakonsen DL, Heider M, Witus SR, Zelter A, Beschauner T, MacCoss MJ, Rapé M
Nature 2025 Jul;643(8073):1117-1126

PEBP1 amplifies mitochondrial dysfunction-induced integrated stress response.

Cheng L, Meliala I, Kong Y, Chen J, Proud CG, Björklund M
eLife 2025 Jan 29;13

Putative PINK1/Parkin activators lower the threshold for mitophagy by sensitizing cells to mitochondrial stress.

Rosencrans WM, Lee RW, McGraw L, Horsburgh I, Wang TY, Quan B, Huynh D, Johnston JA, Chan DC, Chou TF
Science advances 2025 Aug 29;11(35):eady0240

Overcoming Cancer Persister Cells by Stabilizing the ATF4 Promoter G-quadruplex.

Xiao C, Li Y, Liu Y, Dong R, He X, Lin Q, Zang X, Wang K, Xia Y, Kong L
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Sep;11(35):e2401748

Mitochondrial rewiring with small-molecule drug-free nanoassemblies unleashes anticancer immunity.

Ren L, Wan J, Li X, Yao J, Ma Y, Meng F, Zheng S, Han W, Wang H
Nature communications 2024 Sep 3;15(1):7664

CaMKII suppresses proteotoxicity by phosphorylating BAG3 in response to proteasomal dysfunction.

Zhang C, Xu H, Tang Q, Duan Y, Xia H, Huang H, Ye D, Bi F
EMBO reports 2024 Oct;25(10):4488-4514

Cytosolic retention of HtrA2 during mitochondrial protein import stress triggers the DELE1-HRI pathway.

Bi PY, Killackey SA, Schweizer L, Arnoult D, Philpott DJ, Girardin SE
Communications biology 2024 Mar 30;7(1):391

The HRI branch of the integrated stress response selectively triggers mitophagy.

Chakrabarty Y, Yang Z, Chen H, Chan DC
Molecular cell 2024 Mar 21;84(6):1090-1100.e6

Deubiquitylase OTUD3 regulates integrated stress response to suppress progression and sorafenib resistance of liver cancer.

Dai H, Wu B, Ge Y, Hao Y, Zhou L, Hong R, Zhang J, Jiang W, Zhang Y, Li H, Zhang L
Cell reports 2024 Jul 23;43(7):114487

Stress response silencing by an E3 ligase mutated in neurodegeneration.

Haakonsen DL, Heider M, Ingersoll AJ, Vodehnal K, Witus SR, Uenaka T, Wernig M, Rapé M
Nature 2024 Feb;626(8000):874-880

Targeting ERK-MYD88 interaction leads to ERK dysregulation and immunogenic cancer cell death.

Virard F, Giraud S, Bonnet M, Magadoux L, Martin L, Pham TH, Skafi N, Deneuve S, Frem R, Villoutreix BO, Sleiman NH, Reboulet J, Merabet S, Chaptal V, Chaveroux C, Hussein N, Aznar N, Fenouil T, Treilleux I, Saintigny P, Ansieau S, Manié S, Lebecque S, Renno T, Coste I
Nature communications 2024 Aug 15;15(1):7037

Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis.

Fu Y, Sacco O, DeBitetto E, Kanshin E, Ueberheide B, Sfeir A
Molecular cell 2023 Oct 19;83(20):3740-3753.e9

A mitochondrial iron-responsive pathway regulated by DELE1.

Sekine Y, Houston R, Eckl EM, Fessler E, Narendra DP, Jae LT, Sekine S
Molecular cell 2023 Jun 15;83(12):2059-2076.e6

Sublethal cytochrome c release generates drug-tolerant persister cells.

Kalkavan H, Chen MJ, Crawford JC, Quarato G, Fitzgerald P, Tait SWG, Goding CR, Green DR
Cell 2022 Sep 1;185(18):3356-3374.e22

Viperin triggers ribosome collision-dependent translation inhibition to restrict viral replication.

Hsu JC, Laurent-Rolle M, Pawlak JB, Xia H, Kunte A, Hee JS, Lim J, Harris LD, Wood JM, Evans GB, Shi PY, Grove TL, Almo SC, Cresswell P
Molecular cell 2022 May 5;82(9):1631-1642.e6

Cytoplasmic proteotoxicity regulates HRI-dependent phosphorylation of eIF2α via the Hsp70-Bag3 module.

Patel S, Kumar S, Baldan S, Hesin A, Yaglom J, Sherman MY
iScience 2022 May 20;25(5):104282

DELE1 tracks perturbed protein import and processing in human mitochondria.

Fessler E, Krumwiede L, Jae LT
Nature communications 2022 Apr 6;13(1):1853

Human NLRP1 is a sensor for double-stranded RNA.

Bauernfried S, Scherr MJ, Pichlmair A, Duderstadt KE, Hornung V
Science (New York, N.Y.) 2021 Jan 29;371(6528)

A pathway coordinated by DELE1 relays mitochondrial stress to the cytosol.

Fessler E, Eckl EM, Schmitt S, Mancilla IA, Meyer-Bender MF, Hanf M, Philippou-Massier J, Krebs S, Zischka H, Jae LT
Nature 2020 Mar;579(7799):433-437

Integration of Hippo signalling and the unfolded protein response to restrain liver overgrowth and tumorigenesis.

Wu H, Wei L, Fan F, Ji S, Zhang S, Geng J, Hong L, Fan X, Chen Q, Tian J, Jiang M, Sun X, Jin C, Yin ZY, Liu Q, Zhang J, Qin F, Lin KH, Yu JS, Deng X, Wang HR, Zhao B, Johnson RL, Chen L, Zhou D
Nature communications 2015 Feb 19;6:6239

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 20499-1-AP(EIF2AK1 antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using EIF2AK1 antibody 20499-1-AP at 1:25 dilution and FITC- labeled goat anti-Rabbit IgG (green).
Sample type: cell line