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- Antigen structure
- References [12]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- 20499-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#20499-1-AP, RRID:AB_10697665
- Product name
- EIF2AK1 antibody
- Antibody type
- Polyclonal
- Description
- EIF2AK1 antibody (Cat. #20499-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IHC, WB,ELISA.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Cytosolic retention of HtrA2 during mitochondrial protein import stress triggers the DELE1-HRI pathway.
The HRI branch of the integrated stress response selectively triggers mitophagy.
Stress response silencing by an E3 ligase mutated in neurodegeneration.
Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis.
A mitochondrial iron-responsive pathway regulated by DELE1.
Sublethal cytochrome c release generates drug-tolerant persister cells.
Viperin triggers ribosome collision-dependent translation inhibition to restrict viral replication.
Cytoplasmic proteotoxicity regulates HRI-dependent phosphorylation of eIF2α via the Hsp70-Bag3 module.
DELE1 tracks perturbed protein import and processing in human mitochondria.
Human NLRP1 is a sensor for double-stranded RNA.
A pathway coordinated by DELE1 relays mitochondrial stress to the cytosol.
Integration of Hippo signalling and the unfolded protein response to restrain liver overgrowth and tumorigenesis.
Bi PY, Killackey SA, Schweizer L, Arnoult D, Philpott DJ, Girardin SE
Communications biology 2024 Mar 30;7(1):391
Communications biology 2024 Mar 30;7(1):391
The HRI branch of the integrated stress response selectively triggers mitophagy.
Chakrabarty Y, Yang Z, Chen H, Chan DC
Molecular cell 2024 Mar 21;84(6):1090-1100.e6
Molecular cell 2024 Mar 21;84(6):1090-1100.e6
Stress response silencing by an E3 ligase mutated in neurodegeneration.
Haakonsen DL, Heider M, Ingersoll AJ, Vodehnal K, Witus SR, Uenaka T, Wernig M, Rapé M
Nature 2024 Feb;626(8000):874-880
Nature 2024 Feb;626(8000):874-880
Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis.
Fu Y, Sacco O, DeBitetto E, Kanshin E, Ueberheide B, Sfeir A
Molecular cell 2023 Oct 19;83(20):3740-3753.e9
Molecular cell 2023 Oct 19;83(20):3740-3753.e9
A mitochondrial iron-responsive pathway regulated by DELE1.
Sekine Y, Houston R, Eckl EM, Fessler E, Narendra DP, Jae LT, Sekine S
Molecular cell 2023 Jun 15;83(12):2059-2076.e6
Molecular cell 2023 Jun 15;83(12):2059-2076.e6
Sublethal cytochrome c release generates drug-tolerant persister cells.
Kalkavan H, Chen MJ, Crawford JC, Quarato G, Fitzgerald P, Tait SWG, Goding CR, Green DR
Cell 2022 Sep 1;185(18):3356-3374.e22
Cell 2022 Sep 1;185(18):3356-3374.e22
Viperin triggers ribosome collision-dependent translation inhibition to restrict viral replication.
Hsu JC, Laurent-Rolle M, Pawlak JB, Xia H, Kunte A, Hee JS, Lim J, Harris LD, Wood JM, Evans GB, Shi PY, Grove TL, Almo SC, Cresswell P
Molecular cell 2022 May 5;82(9):1631-1642.e6
Molecular cell 2022 May 5;82(9):1631-1642.e6
Cytoplasmic proteotoxicity regulates HRI-dependent phosphorylation of eIF2α via the Hsp70-Bag3 module.
Patel S, Kumar S, Baldan S, Hesin A, Yaglom J, Sherman MY
iScience 2022 May 20;25(5):104282
iScience 2022 May 20;25(5):104282
DELE1 tracks perturbed protein import and processing in human mitochondria.
Fessler E, Krumwiede L, Jae LT
Nature communications 2022 Apr 6;13(1):1853
Nature communications 2022 Apr 6;13(1):1853
Human NLRP1 is a sensor for double-stranded RNA.
Bauernfried S, Scherr MJ, Pichlmair A, Duderstadt KE, Hornung V
Science (New York, N.Y.) 2021 Jan 29;371(6528)
Science (New York, N.Y.) 2021 Jan 29;371(6528)
A pathway coordinated by DELE1 relays mitochondrial stress to the cytosol.
Fessler E, Eckl EM, Schmitt S, Mancilla IA, Meyer-Bender MF, Hanf M, Philippou-Massier J, Krebs S, Zischka H, Jae LT
Nature 2020 Mar;579(7799):433-437
Nature 2020 Mar;579(7799):433-437
Integration of Hippo signalling and the unfolded protein response to restrain liver overgrowth and tumorigenesis.
Wu H, Wei L, Fan F, Ji S, Zhang S, Geng J, Hong L, Fan X, Chen Q, Tian J, Jiang M, Sun X, Jin C, Yin ZY, Liu Q, Zhang J, Qin F, Lin KH, Yu JS, Deng X, Wang HR, Zhao B, Johnson RL, Chen L, Zhou D
Nature communications 2015 Feb 19;6:6239
Nature communications 2015 Feb 19;6:6239
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 20499-1-AP(EIF2AK1 antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using EIF2AK1 antibody 20499-1-AP at 1:25 dilution and FITC- labeled goat anti-Rabbit IgG (green).
- Sample type
- cell line
