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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NBP1-49972 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-49972, RRID:AB_10012162
- Product name
- Rabbit Polyclonal RNGTT Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references RNA-binding proteins and heat-shock protein 90 are constituents of the cytoplasmic capping enzyme interactome.
Identification of potential drug targets for tuberous sclerosis complex by synthetic screens combining CRISPR-based knockouts with RNAi.
Trotman JB, Agana BA, Giltmier AJ, Wysocki VH, Schoenberg DR
The Journal of biological chemistry 2018 Oct 26;293(43):16596-16607
The Journal of biological chemistry 2018 Oct 26;293(43):16596-16607
Identification of potential drug targets for tuberous sclerosis complex by synthetic screens combining CRISPR-based knockouts with RNAi.
Housden BE, Valvezan AJ, Kelley C, Sopko R, Hu Y, Roesel C, Lin S, Buckner M, Tao R, Yilmazel B, Mohr SE, Manning BD, Perrimon N
Science signaling 2015 Sep 8;8(393):rs9
Science signaling 2015 Sep 8;8(393):rs9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RNGTT Antibody [NBP1-49972] - Detection of Human and Mouse RNGTT by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-RNGTT antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. RNGTT was also immunoprecipitated by rabbit anti-RNGTT antibody which recognizes a downstream epitope. For blotting immunoprecipitated RNGTT was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RNGTT Antibody [NBP1-49972] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RNGTT used at a dilution of 1:200 (1ug/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate
