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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [3]
- Other assay [1]
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- Product number
- PA5-34624 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRIM23 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: HeLaS3. Predicted reactivity: Rhesus Monkey (100%). IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.67 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Whole exome sequencing reveals the maintained polyclonal nature from primary to metastatic malignant peripheral nerve sheath tumor in two patients with NF1.
Godec A, Jayasinghe R, Chrisinger JSA, Prudner B, Ball T, Wang Y, Srihari D, Kaushal M, Dietz H, Zhang X, Pekmezci M, Dahiya S, Tao Y, Luo J, Van Tine BA, Ding L, Gutmann DH, Hirbe AC
Neuro-oncology advances 2020 Jul;2(Suppl 1):i75-i84
Neuro-oncology advances 2020 Jul;2(Suppl 1):i75-i84
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells using anti-TRIM23 polyclonal antibody (Product # PA5-34624) (Green) at a 1:500 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of TRIM23 using TRIM23 Polyclonal Antibody (Product # PA5-34624). Samples used were HCT116 cells mock (left) and cells treated with 30 µM Cisplatin for 24 hrs (right) fixed in 4% paraformaldehyde at RT for 15 min. Green: TRIM23 Polyclonal Antibody (Product # PA5-34624) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of TRIM23 in paraformaldehyde-fixed HeLa cells using TRIM23 Polyclonal Antibody (Product # PA5-34624) (Green) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3. Identification of TRIM Family as potential drivers of progression and survival. (A) Kaplan-Meier survival curves for TRIM23 expression in sarcoma (SARC), glioblastoma (GBM), and breast carcinoma (BRCA) from TCGA GEPIA database. (B) Kaplan-Meier survival curves for TRIM23 expression from a cohort staining analysis of MPNST patients from Washington University in St. Louis and UCSF ( P = .15; n = 39). (C) Representative examples of TRIM23 staining in MPNST.
