MA1-19285

antibody from Invitrogen Antibodies

Targeting: SIT1 SIT

Immunoprecipitation

Antibody data

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Validation data

Product number: MA1-19285 - Provider product page
Provider: Invitrogen Antibodies
Product name: SIT Monoclonal Antibody (SIT-01)
Antibody type: Monoclonal
Antigen: Recombinant protein fragment
Description: This antibody reacts with murine SIT. Western Blot: Reducing conditions.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: SIT-01
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: 4°C, do not freeze

SIT1 protein structure - MA1-19285 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blotting analysis of human SIT using mouse monoclonal antibody SIT-01 on lysates of Molt-4 and HEK-293T cells under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2µg/mL of mouse anti-SIT Monoclonal antibody (Product # MA1-192850) followed by IRDye800-conjugated anti-mouse secondary antibody. SIT was detected around 36kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of SIT using SIT Monoclonal Antibody (SIT-01) (Product # MA1-19285). Analysis was performed on whole cell extracts (RIPA lysis buffer) of Ramos and Jurkat cell lines, mixed and heated (100°C, 5 min) with reducing (2-mercaptoethanol) SDS-loading buffer. Samples were resolved using 10% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed simultaneously with mouse IgG1 monoclonal antibody SIT-01 (2 µg/mL), and rat IgG2a anti-tubulin monoclonal antibody (1 µg/mL) used as the loading control. Subclass-specific secondary antibodies Goat-anti-Rat IgG (green) and Goat-anti-Mouse IgG (red) were used for multiplex fluorescent Western blot detection. SIT was detected at ~32 kDa in tested cell lines.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of SIT using SIT Monoclonal Antibody (SIT-01) (Product # MA1-19285). The specificity of SIT-01 antibody was assessed by comparing binding signals in HEK293T cells overexpressing the target SIT protein to wild type cells (control) with low level of endogenous protein expression. Western blotting analysis was performed on whole cell extracts (urea lysis buffer) of transfected and control cells, mixed and heated (100°C, 5 min) with reducing (2-mercaptoethanol) SDS-loading buffer. Samples were resolved using 10% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed simultaneously with mouse IgG1 monoclonal antibody (2 µg/mL), and rat IgG2a anti-tubulin monoclonal antibody (1 µg/mL) used as the loading control. Subclass-specific secondary antibodies Goat-anti-Rat IgG (green) and Goat-anti-Mouse IgG (red) were used for multiplex fluorescent Western blot detection.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-SIT (SIT-01) purified Monoclonal antibody (Product # MA1-19285) (concentration in sample 9 µg/mL, GAM APC).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Separation of human CD3 negative SIT positive lymphocytes (red-filled) from CD3 negative SIT negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral human whole blood stained using anti-SIT (SIT-01) purified Monoclonal antibody (Product # MA1-19285) (concentration in sample 9 µg/mL, GAM APC).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-SIT (SIT-01) purified Monoclonal antibody (Product # MA1-19285) (concentration in sample 9 µg/mL, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ using a dilution of 20 µL reagent/100 µL of peripheral whole blood.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-SIT (SIT-01) purified Monoclonal antibody (Product # MA1-19285) (concentration in sample 9 µg/mL, GAM APC).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Separation of human CD3 negative SIT positive lymphocytes (red-filled) from CD3 negative SIT negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral human whole blood stained using anti-SIT (SIT-01) purified Monoclonal antibody (Product # MA1-19285) (concentration in sample 9 µg/mL, GAM APC).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-SIT (SIT-01) purified Monoclonal antibody (Product # MA1-19285) (concentration in sample 9 µg/mL, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ using a dilution of 20 µL reagent/100 µL of peripheral whole blood.