Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [3]
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Validation data
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- Product number
- MA1-19285 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SIT Monoclonal Antibody (SIT-01)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Description
- This antibody reacts with murine SIT. Western Blot: Reducing conditions.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- SIT-01
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blotting analysis of human SIT using mouse monoclonal antibody SIT-01 on lysates of Molt-4 and HEK-293T cells under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2µg/mL of mouse anti-SIT Monoclonal antibody (Product # MA1-192850) followed by IRDye800-conjugated anti-mouse secondary antibody. SIT was detected around 36kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blotting analysis of human SIT using mouse monoclonal antibody SIT-01 on lysates of Molt-4 and HEK-293T cells under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2µg/mL of mouse anti-SIT Monoclonal antibody (Product # MA1-192850) followed by IRDye800-conjugated anti-mouse secondary antibody. SIT was detected around 36kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-SIT (SIT-01) purified Monoclonal antibody (Product # MA1-19285) (concentration in sample 9 µg/mL, GAM APC).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Separation of human CD3 negative SIT positive lymphocytes (red-filled) from CD3 negative SIT negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral human whole blood stained using anti-SIT (SIT-01) purified Monoclonal antibody (Product # MA1-19285) (concentration in sample 9 µg/mL, GAM APC).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-SIT (SIT-01) purified Monoclonal antibody (Product # MA1-19285) (concentration in sample 9 µg/mL, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ using a dilution of 20 µL reagent/100 µL of peripheral whole blood.