Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
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Validation data
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- Product number
- NBP2-22339 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Kremen-2 Antibody
- Antibody type
- Polyclonal
- Description
- Peptide affinity purified. Multiple isoforms of KREMEN2 are known to exist. KREMEN2 antibody is predicted to not cross-react with other Kremen protein family members.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Kremen-2 Antibody [NBP2-22339] - Analysis of KREMEN2 in HeLa cell lysate with KREMEN2 antibody at (A) 1 and (B) 2 ug/mL.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Kremen-2 Antibody [NBP2-22339] - Western Blot Validation in Mouse Spleen Tissue Lysate. Loading: 15 ug of lysates per lane. Antibodies: KREMEN2 NBP2-22339 (Lane 1: 1 ug/mL and Lane 2: 2 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Kremen-2 Antibody [NBP2-22339] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: KREMEN2, NBP2-22339 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Kremen-2 Antibody [NBP2-22339] - Independent Antibody Validation via Protein Expression Profile in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: Kremen-2 [NBP2-22339] (2 ug/ml), Kremen-2 antibody from Company B (1 ug/ml) and beta-actin [NBP1-76692] (2 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.