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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- MAB9494-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Fc mu R/FAIM3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Fc mu R/FAIM3 in direct ELISAs. Detects human, mouse, and rat Fc mu R/FAIM3 in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
O60667- Isotype
- IgG
- Antibody clone number
- 992338
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat Fc mu R/FAIM3 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, A20 mouse B cell lymphoma cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat Fc mu R/FAIM3 Monoclonal Antibody (Catalog # MAB9494) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Fc mu R/FAIM3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of FAIM3 in HEK293 Human Cell Line Transfected with Human FAIM3 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human FAIM3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human/Mouse/Rat FAIM3 Monoclonal Antibody (Catalog # MAB9494) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.
