Explore
Validate
Learn
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA3-028 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- OPRK1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- IHC (P) analysis shows positive staining of KOR in human brain. WB analysis shows KOR in glycoprotein-enriched fractions from mock transfected or KOR overexpressing 293 cells. While KOR has a theoretical MW of ~ 43kD, the observed band in a WB runs at ~60kD due to enrichment of the glycosylated receptor.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of KOR was performed on human brain tissue. To expose target proteins, antigen retrieval was performed by microwaving tissues for 20 minutes in 10mM sodium citrate buffer (pH 6.0). Tissue slides were probed with a KOR polyclonal antibody (Product # PA3-028) at a dilution of 1:3000, overnight at 4C in a humidified chamber. Tissues were washed, and detection was performed using an ABC kit composed of biotinylated goat anti-rabbit IgG, peroxidase-conjugated avidin, and 3-amino-9-ethylcarbazole (AEC) substrate in acetate buffer. Tissues were counterstained with hematoxylin and dehydrated to prep for mounting.
