PA5-51665

antibody from Invitrogen Antibodies

Targeting: FBLN2

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-51665

Provider

Invitrogen Antibodies

Product name

Fibulin 2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: TLGSFHCYKA LTCEPGYALK DGECEDVDEC AMGTHTCQPG FLCQNTKGSF YCQARQRCMD GFLQDPEGNC VDINECTSLS EPCRPGFSCI NTVGSYTCQR NPLICARGYH ASDDGTKCVD VNE

Concentration

0.15 mg/mL

References

Submitted references

Fibulin-2 expression associates with vascular invasion and patient survival in breast cancer.

Klingen TA, Chen Y, Aas H, Wik E, Akslen LA
PloS one 2021;16(4):e0249767

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Flow-induced Reorganization of Laminin-integrin Networks Within the Endothelial Basement Membrane Uncovered by Proteomics.

Béguin EP, Janssen EFJ, Hoogenboezem M, Meijer AB, Hoogendijk AJ, van den Biggelaar M
Molecular & cellular proteomics : MCP 2020 Jul;19(7):1179-1192

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of Fibulin 2 in human cell line BJ using a Fibulin 2 Polyclonal Antibody (Product # PA5-51665) shows localization to plasma membrane.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Fibulin 2 in human urinary bladder and liver tissues using Fibulin 2 Polyclonal Antibody (Product # PA5-51665). Corresponding FBLN2 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Fibulin 2 in human urinary bladder using Fibulin 2 Polyclonal Antibody (Product # PA5-51665) shows high expression.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Fibulin 2 in human liver using Fibulin 2 Polyclonal Antibody (Product # PA5-51665) shows low expression as expected.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 1 Histological images of perivascular fibulin-2 expression by immuno-histochemistry. Histological images of tumour tissue with absent or faint/barely perceptible peripheral staining in tumour vessels (A), weak to moderate staining (B), or strong/thick staining (C) for fibulin-2 in vessels at the tumour periphery (x200). Scale bars are shown on each image.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 3. Flow induces remodeling of the endothelial extracellular matrix. A , Proteome and ( B ) cell surface proteome data of identified laminins. T or CT represents the enzyme used for protein digestion. The depicted numbers represent the biotin-modified lysine residue. C , Schematic representation of laminin 411 with the linker region between domains LG3 and LG4. The blue lysine residues were found to be decreased under flow conditions (as depicted in panel B ). D , Immunoblot analysis of LAMA4 in ECs exposed to flow over time. alpha-tubulin was used as a loading control. E , Protein distribution of LAMA5 and LAMA4 after flow-exposure. Green represents LAMA4 or LAMA5, blue is HOECHST. The scale bar is 10 mum. Depicted are maximum intensity projections. F , Proteome and ( G ) cell surface proteome data of identified fibulins. H , Immunofluorescence analysis of EFEMP1 and FBLN2 abundance and distribution affected by flow. Red is VE-cadherin, blue is HOECHST. Depicted are maximum intensity projections. Micrographs are representative for 3 independent experiments. The scale bar is 10 mum.