PA5-21640

antibody from Invitrogen Antibodies

Targeting: FBLN2

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21640

Provider

Invitrogen Antibodies

Product name

Fibulin 2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, HeLa, HepG2. Predicted reactivity: Mouse (86%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.39 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

An Investigation of Fibulin-2 in Hypertrophic Cardiomyopathy.

Ibrahim AM, Roshdy M, Elshorbagy S, Hosny M, Halawa S, Yehia D, Elfawy HA, Eldessouki A, Mohamed F, Ellithy A, Abdelfattah M, Elsawy A, Elkhatib M, Allouba M, Elguindy A, Aguib Y, Yacoub M
International journal of molecular sciences 2020 Sep 29;21(19)

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 FBLN2 expression and localization in HCM myectomy tissues along with Col IV and TGFbeta1. ( A ) A representative transmural section of a myectomy specimen stained with picro-Sirius red and shows fibrosis distribution in the myocardium and sub-endocardium. Scale bar is 1 mm. ( B ) Picro-sirius red and immunohistochemical analysis of HCM tissues at the myocardium and sub-endocardial ( n = 79), compared to controls ( n = 9), showing FBLN2, Col IV, and TGFbeta1 expression. Scale bars are 100 (overview) and 20 um (magnified insets). ( C ) Confocal microscopy imaging shows Vim+ and SMA+ cells (red) infiltrating the FBLN2 sheath (green) at the sub-endocardial region (representative of 3 HCMs vs. 3 controls). Scale bars are 20 um.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 Elevated expression of FBLN2 in HCM myectomy tissues and cultured fibroblasts. ( A ) A representative Immunoblot for HCM and ctrl tissues shows total FBLN2 protein expression normalized to Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) expression. ( B ) Bar plot shows a significant difference ( P = 0.0009) between mean FBLN2 expression in HCM tissues ( n = 44) compared to ctrl tissues ( n = 7). ( C ) A representative Immunoblot for cultured HCM and ctrl fibroblasts shows total FBLN2 protein expression normalized to GAPDH expression. ( D ) Bar plot shows an elevated level of FBLN2 in cultured HCM fibroblasts ( n = 34) compared to ctrl fibroblasts ( n = 5). E and F: Confocal microscope imaging of cultured HCM ( n = 3) and control fibroblasts ( n = 3), shows FBLN2 expression pattern and localization. Cells were co-stained with FBLN2 and vimentin (mesenchymal fibroblasts marker) ( E ), and FBLN2 with smooth muscle actin (myofibroblasts marker) ( F ). Scale bars are 20 um.