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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AF5207 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal CCR4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human and mouse CCR4 in direct ELISAs and Western blots.
- Reactivity
- Human, Mouse
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Endogenous expression of matriptase in neural progenitor cells promotes cell migration and neuron differentiation.
Fang JD, Chou HC, Tung HH, Huang PY, Lee SL
The Journal of biological chemistry 2011 Feb 18;286(7):5667-79
The Journal of biological chemistry 2011 Feb 18;286(7):5667-79
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human/Mouse CCR4 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and EL-4 mouse lymphoblast cell line. PVDF Membrane was probed with 1 µg/mL of Mouse CCR4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5207) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CCR4 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8
