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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [15]
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- Validations
- Flow cytometry [1]
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- Product number
- MAB1567-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CCR4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Stains human CCR4-transfected cell lines but not the corresponding parent cell lines. Also detects CCR4 on human PBLs and platelets.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P51679- Isotype
- IgG
- Antibody clone number
- 205410
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Desensitization and treatment with APRIL/BLyS blockade in rodent kidney transplant model.
Optimization of mass cytometry sample cryopreservation after staining.
CD20+ B cell depletion alters T cell homing.
Functional diversity of human vaginal APC subsets in directing T-cell responses.
Long-term Vaccination with Multiple Peptides Derived from Cancer-Testis Antigens Can Maintain a Specific T-cell Response and Achieve Disease Stability in Advanced Biliary Tract Cancer.
Identification of human NK17/NK1 cells.
Mouse CCL8, a CCR8 agonist, promotes atopic dermatitis by recruiting IL-5+ T(H)2 cells.
Controlled delivery of T-box21 small interfering RNA ameliorates autoimmune alopecia (Alopecia Areata) in a C3H/HeJ mouse model.
Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells.
The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities.
CD molecules 2006--human cell differentiation molecules.
Cutting edge: nonproliferating mature immune cells form a novel type of organized lymphoid structure in idiopathic pulmonary fibrosis.
Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
Classification of distinct subtypes of peripheral T-cell lymphoma unspecified, identified by chemokine and chemokine receptor expression: Analysis of prognosis.
A CCR2-V64I polymorphism affects stability of CCR2A isoform.
Bath NM, Ding X, Wilson NA, Verhoven BM, Boldt BA, Sukhwal A, Reese SR, Panzer SE, Djamali A, Redfield RR 3rd
PloS one 2019;14(2):e0211865
PloS one 2019;14(2):e0211865
Optimization of mass cytometry sample cryopreservation after staining.
Sumatoh HR, Teng KW, Cheng Y, Newell EW
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2017 Jan;91(1):48-61
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2017 Jan;91(1):48-61
CD20+ B cell depletion alters T cell homing.
Kap YS, van Driel N, Laman JD, Tak PP, 't Hart BA
Journal of immunology (Baltimore, Md. : 1950) 2014 May 1;192(9):4242-53
Journal of immunology (Baltimore, Md. : 1950) 2014 May 1;192(9):4242-53
Functional diversity of human vaginal APC subsets in directing T-cell responses.
Duluc D, Gannevat J, Anguiano E, Zurawski S, Carley M, Boreham M, Stecher J, Dullaers M, Banchereau J, Oh S
Mucosal immunology 2013 May;6(3):626-38
Mucosal immunology 2013 May;6(3):626-38
Long-term Vaccination with Multiple Peptides Derived from Cancer-Testis Antigens Can Maintain a Specific T-cell Response and Achieve Disease Stability in Advanced Biliary Tract Cancer.
Aruga A, Takeshita N, Kotera Y, Okuyama R, Matsushita N, Ohta T, Takeda K, Yamamoto M
Clinical cancer research : an official journal of the American Association for Cancer Research 2013 Apr 15;19(8):2224-31
Clinical cancer research : an official journal of the American Association for Cancer Research 2013 Apr 15;19(8):2224-31
Identification of human NK17/NK1 cells.
Pandya AD, Al-Jaderi Z, Høglund RA, Holmøy T, Harbo HF, Norgauer J, Maghazachi AA
PloS one 2011;6(10):e26780
PloS one 2011;6(10):e26780
Mouse CCL8, a CCR8 agonist, promotes atopic dermatitis by recruiting IL-5+ T(H)2 cells.
Islam SA, Chang DS, Colvin RA, Byrne MH, McCully ML, Moser B, Lira SA, Charo IF, Luster AD
Nature immunology 2011 Feb;12(2):167-77
Nature immunology 2011 Feb;12(2):167-77
Controlled delivery of T-box21 small interfering RNA ameliorates autoimmune alopecia (Alopecia Areata) in a C3H/HeJ mouse model.
Nakamura M, Jo J, Tabata Y, Ishikawa O
The American journal of pathology 2008 Mar;172(3):650-8
The American journal of pathology 2008 Mar;172(3):650-8
Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells.
Kang SG, Lim HW, Andrisani OM, Broxmeyer HE, Kim CH
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):3724-33
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):3724-33
The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities.
Ponte AL, Marais E, Gallay N, Langonné A, Delorme B, Hérault O, Charbord P, Domenech J
Stem cells (Dayton, Ohio) 2007 Jul;25(7):1737-45
Stem cells (Dayton, Ohio) 2007 Jul;25(7):1737-45
CD molecules 2006--human cell differentiation molecules.
Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Bühring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X
Journal of immunological methods 2007 Jan 30;319(1-2):1-5
Journal of immunological methods 2007 Jan 30;319(1-2):1-5
Cutting edge: nonproliferating mature immune cells form a novel type of organized lymphoid structure in idiopathic pulmonary fibrosis.
Marchal-Sommé J, Uzunhan Y, Marchand-Adam S, Valeyre D, Soumelis V, Crestani B, Soler P
Journal of immunology (Baltimore, Md. : 1950) 2006 May 15;176(10):5735-9
Journal of immunology (Baltimore, Md. : 1950) 2006 May 15;176(10):5735-9
Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
Clinical immunology (Orlando, Fla.) 2006 Aug;120(2):220-31
Clinical immunology (Orlando, Fla.) 2006 Aug;120(2):220-31
Classification of distinct subtypes of peripheral T-cell lymphoma unspecified, identified by chemokine and chemokine receptor expression: Analysis of prognosis.
Ohshima K, Karube K, Kawano R, Tsuchiya T, Suefuji H, Yamaguchi T, Suzumiya J, Kikuchii M
International journal of oncology 2004 Sep;25(3):605-13
International journal of oncology 2004 Sep;25(3):605-13
A CCR2-V64I polymorphism affects stability of CCR2A isoform.
Nakayama EE, Tanaka Y, Nagai Y, Iwamoto A, Shioda T
AIDS (London, England) 2004 Mar 26;18(5):729-38
AIDS (London, England) 2004 Mar 26;18(5):729-38
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CCR4 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A) and either (A) Mouse Anti-Human CCR4 Monoclonal Antibody (Catalog # MAB1567) or (B) Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
