46-1949-41

antibody from Invitrogen Antibodies
Targeting: CCR4 CC-CKR-4, CD194, ChemR13, CKR4, CMKBR4, k5-5
Provider product page for 46-1949-41

Antibody data

Product number
46-1949-41 - Provider product page
Provider
Invitrogen Antibodies
Product name
Anti-CD194 (CCR4) Monoclonal Antibody (D8SEE), PerCP-eFluor 710, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: This D8SEE monoclonal antibody reacts with human CD194 (CCR4). CD194, also known as C-C Chemokine Receptor 4 (CCR4) is a member of the G protein-coupled receptor family, a large family characterized by seven transmembrane alpha helical domains. The ligands for CCR4 are CCL17 (TARC) and CCL22 (MDC), which direct the migration of target cells. CCR4 and its ligands, along with the other receptors CCR10 and CLA, are particularly important for the recruitment of T cells into the skin, where they may also play a role in adhesion and extravasation. Although CCR4 expression was originally believed to be restricted to Th2 cells, it has since been observed in some subsets of Th17, Treg, and Th1 cells. It is not expressed by naive T cells, indicating a role in memory or effector function. Other cells that express CCR4 are activated NK cells, platelets, monocytes, and basophils. Applications Reported: This D8SEE antibody has been reported for use in flow cytometric analysis. Applications Tested: This D8SEE antibody has been pre-titrated and tested by flow cytometric anaylis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
D8SEE
Vial size
25 Tests
Concentration
5 µL/Test
Storage
4° C, store in dark, DO NOT FREEZE!
CCR4 protein structure - 46-1949-41 shown in red.