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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00050862-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00050862-M01, RRID:AB_547761
- Product name
- Mouse Monoclonal RNF141 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RNF141 - ring finger protein 141
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RNF141 Antibody (6D9) [H00050862-M01] - Analysis of RNF141 over-expressed 293 cell line, cotransfected with RNF141 Validated Chimera RNAi ( Cat # H00050862-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RNF141 monoclonal antibody (M01), clone 6D9 (Cat # H00050862-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RNF141 Antibody (6D9) [H00050862-M01] - Analysis of RNF141 expression in transfected 293T cell line by RNF141 monoclonal antibody (M01), clone 6D9.Lane 1: RNF141 transfected lysate(25.5 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RNF141 Antibody (6D9) [H00050862-M01] - RNF141 monoclonal antibody (M01), clone 6D9 Analysis of RNF141 expression in A-431.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: RNF141 Antibody (6D9) [H00050862-M01] - Detection limit for recombinant GST tagged RNF141 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RNF141 Antibody (6D9) [H00050862-M01] - Analysis of monoclonal antibody to RNF141 on formalin-fixed paraffin-embedded human testis. Antibody concentration 0.3 ug/ml.
