AP8849c

antibody from Abcepta

Targeting: OLR1 CLEC8A, LOX-1, SCARE1

Antibody data

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Validation data

Product number: AP8849c - Provider product page
Provider: Abcepta
Proper citation: Abgent Cat#AP8849c, RRID:AB_10552784
Product name: OLR1 Antibody (Center)
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 400 µl
Concentration: 0.5 mg/ml
Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

OLR1 protein structure - AP8849c shown in red.

Supportive validation

Submitted by: Abcepta (provider)
Main image
Experimental details: "Western blot analysis of lysates from Hela cell line , human liver and placenta tissue lysate(from left to right), using OLR1 Antibody (Center)(Cat. #AP8849c). AP8849c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane."
Primary Ab dilution: 1:1000

Supportive validation

Submitted by: Abcepta (provider)
Main image
Experimental details: "Western blot analysis of lysate from HUVEC cell line, using OLR1 Antibody (Center)(Cat. #AP8849c). AP8849c was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug."
Primary Ab dilution: 1:1000

Supportive validation

Submitted by: Abcepta (provider)
Main image
Experimental details: Western blot analysis of OLR1 Antibody (Center) (Cat. #AP8849c) in HL-60 cell line lysates (35ug/lane).OLR1 (arrow) was detected using the purified Pab.
Primary Ab dilution: 1:1000

Supportive validation

Submitted by: Abcepta (provider)
Main image
Experimental details: Western blot analysis of OLR1 (arrow) using rabbit polyclonal OLR1 Antibody (Center) (Cat. #AP8849c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the OLR1 gene (Lane 2) .
Primary Ab dilution: 1:1000

Supportive validation

Submitted by: Abcepta (provider)
Main image
Experimental details: "Western blot analysis of lysates from A549, HUVEC cell line and human liver, placenta tissue lysate(from left to right), using OLR1 Antibody (Center)(Cat. #AP8849c). AP8849c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane."
Primary Ab dilution: 1:1000