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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-95750 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LOX-1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MQLSQVSDLL TQEQANLTHQ KKKLEGQISA RQQAEEASQE SENELKEMIE TLARKLNEKS KEQMELHHQN LNLQETLKRV ANCSAPCPQD WIWHGENCYL FSSGSFNWEK SQEKCLSLDA KLLKINSTAD LDFIQQAISY SSFPFWMGLS RRNPSYPWLW EDGSPLMPHL FRVRGAVSQT YPSGTCAYIQ RGAVYAENCI LAAFSICQKK ANLRAQ; Positive Samples: OVCAR3, HeLa, HepG2; Cellular Location: Cell membrane, Lipid-anchor, Membrane raft, Single-pass type II membrane protein
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.30 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Exosomes released from mesenchymal stem cells overexpressing microRNA-30e ameliorate heart failure in rats with myocardial infarction.
Pu L, Kong X, Li H, He X
American journal of translational research 2021;13(5):4007-4025
American journal of translational research 2021;13(5):4007-4025
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of LOX-1 in extracts of various cell lines using LOX-1 Polyclonal Antibody (Product # PA5-95750) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of LOX-1 in paraffin-embedded rat brain using LOX-1 Polyclonal Antibody (Product # PA5-95750) at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of LOX-1 in paraffin-embedded human kidney using LOX-1 Polyclonal Antibody (Product # PA5-95750) at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of LOX-1 in paraffin-embedded mouse intestine using LOX-1 Polyclonal Antibody (Product # PA5-95750) at a dilution of 1:200.
