Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-72815 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SOX17 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SOX17 in HepG2 cell lysate with SOX17 Polyclonal Antibody (Product # PA5-72815) at (A) 0.5 and (B) 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-SOX17 Polyclonal Antibody (Product # PA5-72815) and a 44 kDa band corresponding to Transcription factor SOX-17 was observed in iPSC differentiated to definitive endoderm and OVCAR-3 when compared to other cell lines. Nuclear enriched extracts (30 µg lysate) of iPSC (Lane 1), iPSC differentiated to definitive endoderm (Lane 2), COS-7 (Lane 3), Hep G2 (Lane 4), OVCAR-3 (Lane 5), IMR-32 (Lane 6), HUVEC (Lane 7) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:6000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of SOX17 in mouse liver tissue with SOX17 Polyclonal Antibody (Product # PA5-72815) at 20 µg/mL.