TA500281

antibody from OriGene

Targeting: SOX17

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500281

Provider

OriGene

Proper citation

OriGene Cat#TA500281, RRID:AB_2195642

Product name

Anti-Sox17 mouse monoclonal antibody, clone OTI2B6 (formerly 2B6)

Antibody type

Monoclonal

Description

Anti-Sox17 mouse monoclonal antibody, clone OTI2B6 (formerly 2B6)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

SOX17

Isotype

IgG

Antibody clone number

OTI2B6

Vial size

100 µl

Concentration

0.15 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOX17 (RC220888, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOX17 .

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SOX17 monoclonal antibody.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-SOX17 mouse monoclonal antibody (TA500281) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SOX17 (RC220888).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-SOX17 mouse monoclonal antibody. (TA500281). Dilution Factor: 1:50; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SOX17 mouse monoclonal antibody. (TA500281). Dilution Factor: 1:50; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.

Validation comment

IHC