MA5-24885

antibody from Invitrogen Antibodies

Targeting: SOX17

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

MA5-24885

Provider

Invitrogen Antibodies

Product name

SOX17 Monoclonal Antibody (OTI3B10)

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

OTI3B10

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Single-cell atlas unveils cellular heterogeneity and novel markers in human neonatal and adult intervertebral discs.

Jiang W, Glaeser JD, Salehi K, Kaneda G, Mathkar P, Wagner A, Ho R, Sheyn D
iScience 2022 Jul 15;25(7):104504

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SOX17 in HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK cells using 10 µg per lane. Samples were probed with SOX17 (Product # MA5-24885) monoclonal antibody at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SOX17 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with SOX17 (Product # MA5-24885) monoclonal antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-SOX17 Monoclonal Antibody (OTI3B10) (Product # MA5-24885) and a 44kDa band corresponding to Transcription factor SOX-17 was observed in iPSC differentiated to definitive endoderm and OVCAR-3 when compared to other cell lines. Nuclear enriched extracts (30 µg lysate) of iPSC (Lane 1), iPSC differentiated to definitive endoderm (Lane 2), COS-7 (Lane 3), Hep G2 (Lane 4), OVCAR-3 (Lane 5), IMR-32 (Lane 6), HUVEC (Lane 7) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2500 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:6000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SOX17 in KhES-3 cells. Cells were probed with a IdU monoclonal antibody (Product # MA5-24885).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SOX17 in KhES-3 cells. Cells were probed with a IdU monoclonal antibody (Product # MA5-24885).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SOX17 in KhES-3 cells. Cells were probed with a IdU monoclonal antibody (Product # MA5-24885).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SOX17 in KhES-3 cells. Cells were probed with a IdU monoclonal antibody (Product # MA5-24885).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Transcription factor SOX-17 was performed using 70% confluent log phase iPSC and iPSC differentiated to definitive endoderm cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SOX17 Monoclonal Antibody (OTI3B10) (Product # MA5-24885) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control iPSC which shows no signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.