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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [4]
- Flow cytometry [1]
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- Product number
- CF500044 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Sox17 Monoclonal Antibody (OTI3B10), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI3B10
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOX17 (RC220888, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-SOX17. Positive lysates LY411669 (100 µg) and LC411669 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10 µg) from 7 different cell lines by using anti-SOX17 monoclonal antibody at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-SOX17 mouse monoclonal antibody (TA500044) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SOX17(RC220888).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure from citation: Immunofluorescence of SOX17 protein level by using anti-SOX17 antibody in human KhES-3 cells. View Citation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HepG2 cells using anti-SOX17 mouse monoclonal antibody (TA500044).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HT29 cells using anti-SOX17 mouse monoclonal antibody (TA500044).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC220888 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SOX17 antibody (TA500044), and then analyzed by flow cytometry.
