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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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- Product number
- CF500096 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Sox17 Monoclonal Antibody (OTI2G8), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI2G8
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOX17 (RC220888, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-SOX17. Positive lysates LY411669 (100 µg) and LC411669 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35 µg) from 9 different cell lines by using anti-SOX17 monoclonal antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of A549 cells using anti-SOX17 mouse monoclonal antibody (TA500096).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-SOX17 mouse monoclonal antibody (TA500096) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SOX17(RC220888).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of A549 cells using anti-SOX17 mouse monoclonal antibody (TA500096).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure from citation: Corresponding conventional and immunhistochemical stains of human seminoma and embryonal carcinoma by using anti-SOX17 antibody. Seminoma: positivity for SOX17 (Left); Embryonal carcinoma: negative staining for SOX17 (Right). Dilution: 1:2000 View Citation
