IC19241G

antibody from R&D Systems

Targeting: SOX17

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC19241G

Provider

R&D Systems

Product name

Human SOX17 Alexa Fluor® 488-conjugated Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human SOX17 in direct ELISAs.

Reactivity

Human

Host

Mouse

Conjugate

Green dye

Antigen sequence

Q9H6I2


Isotype

IgG

Antibody clone number

614013

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

SOX17 in BG01V Human Embryonic Stem Cells. SOX17 was detected in immersion fixed definitive endoderm-differentiated BG01V human embryonic stem cells using Mouse Anti-Human SOX17 Alexa Fluor® 488-conjugated Monoclonal Antibody (green, left panel; Catalog # IC19241G) at 10 µg/mL for 3 hours at room temperature and counterstained with DAPI (blue, right panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of SOX17 in Definitive Endoderm-differentiated BG01V Human Stem Cells by Flow Cytometry. Definitive endoderm-differentiated BG01V human embryonic stem cells were stained with Mouse Anti-Human SOX17 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC19241G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.